Anne of Ostfriesland (June 26, 1562 – April 21, 1621) was the eldest daughter of Count Edzard II of East Frisia and his wife, Catherine Vasa, daughter of Gustav I of Sweden.

Anne married three times:

First, on July 12, 1583, in Heidelberg, to the Elector Palatine, Louis VI. He died on October 22 of the same year.
Second, Anne remarried on December 21, 1585, Ernest Frederick, Margrave of Baden-Durlach. He died April 14, 1604.
Third, Anne married on March 7, 1617, in Grabow, Julius Henry, later Duke of Saxe-Lauenburg. The duke was 26 years younger than his wife and outlived her by many decades, dying on November 20, 1665.

Anne had no living children by any of her husbands. She was buried in the Church of the Holy Spirit, Heidelberg, but her grave is not preserved.