This is the progression of world record improvements of the 400 metres W65 division of Masters athletics.
- Key
Incomplete information Pending
| Hand | Auto | Athlete | Nationality | Birthdate | Age | Location | Date | Ref |
|---|---|---|---|---|---|---|---|---|
| 1:07.23 | Elizabeth Deak |  United States
|
24 February 1958 | 65 years, 147 days | Greensboro | 21 July 2023 | [1] | |
| 1:08.08 | Karla Del Grande |  Canada
|
27 March 1953 | 66 years, 107 days | Toronto | 12 July 2019 | ||
| 1:08.21 | Diane Palmason | Canada
|
15 March 1938 | 65 years, 146 days | Eugene | 8 August 2003 | ||
| 1:11.45 | Carolyn Sue Cappetta | United States
|
1936 | 65 | Brisbane | 13 July 2001 | ||
| 1:13.71 | Anna Mangler |  Germany
|
14 January 1923 | 66 years, 203 days | Eugene | 5 August 1989 | ||
| 1:14.31 | Paula Schneiderhan | Germany
|
16 November 1921 | 66 years, 19 days | Melbourne | 5 December 1987 | ||
| 1:16.7 | Winifred Audrey Reid |  South Africa
|
1915 | 68 | Germiston | 19 May 1984 |