The von Kossa histological stain is used to quantify mineralization in cell culture and histological sections.
This is a staining method to illustrates mineralization such as calcium and potassium in tissues.[1][2][3][4][5]
It is a precipitation reaction in which silver ions react with phosphate in the presence of acidic material.[6] Photochemical degradation of silver phosphate to silver happens under light illumination.
Additional methods need to be employed to confirm the presence of calcium, such as Alizarin Red S.[citation needed]