The 13th Lambda Literary Awards were held in 2001 to honour works of LGBT literature published in 2000.
| Category | Winner |
|---|---|
| Pioneer Award | Astraea Foundation |
| Category | Winner | Nominated |
|---|---|---|
| Anthologies/Fiction | David Bergman and Karl Woelz, eds., Men on Men 2000 |
|
| Anthologies/Non-Fiction | Noelle Howey and Ellen Samuels, eds., Out of the Ordinary |
|
| Children's/Young Adult | Noelle Howey and Ellen Samuels, eds., Out of the Ordinary |
|
| Drama | John Cameron Mitchell and Stephen Trask, Hedwig and the Angry Inch |
|
| Gay Biography/Autobiography | Douglas Murray, Bosie: A Biography of Lord Alfred Douglas |
|
| Gay Fiction | K. M. Soehnlein, The World of Normal Boys |
|
| Gay Mystery | John Morgan Wilson, The Limits of Justice |
|
| Gay Poetry | Carl Phillips, Pastoral |
|
| Gay Studies | James J. Berg and Chris Freeman, The Isherwood Century |
|
| Humor | David Sedaris, Me Talk Pretty One Day |
|
| Lesbian Biography/Autobiography | Judith Barrington, Lifesaving |
|
| Lesbian Fiction | Michelle Tea, Valencia |
|
| Lesbian Mystery | Jean Marcy, Mommy Deadest |
|
| Lesbian Poetry | Elena Georgiou, Mercy Mercy Me |
|
| Lesbian Studies | Harmony Hammond, Lesbian Art in America |
|
| Science fiction, fantasy or horror | Jim Grimsley, Kirith Kirin |
|
| Small press | Lauren Sanders, Kamikaze Lust Erasmo Guerra, Between Dances |
|
| Spirituality | Toby Johnson, Gay Spirituality Krandall Kraus and Paul Borja, It's Never About What It's About |
|
| Transgender | David Ebershoff, The Danish Girl |
|
| Visual Arts | Keri Pickett, Faeries |
|