The 2000 All-Big 12 Conference football team consists of American football players chosen as All-Big 12 Conference players for the 2000 Big 12 Conference football season. The conference recognizes two official All-Big 12 selectors: (1) the Big 12 conference coaches selected separate offensive and defensive units and named first- and second-team players (the "Coaches" team);^[1] and (2) a panel of sports writers and broadcasters covering the Big 12 also selected offensive and defensive units and named first- and second-team players (the "Media" team).^[2]

Offensive selections

Quarterbacks

• Josh Heupel, Oklahoma (Coaches-1; Media-1)
• Kliff Kingsbury, Texas Tech (Coaches-2)
• Eric Crouch, Nebraska (Media-2)

Running backs

• Ennis Haywood, Iowa State (Coaches-1; Media-1)
• Hodges Mitchell, Texas (Coaches-1; Media-1)
• Dan Alexander, Nebraska (Coaches-1; Media-2)
• David Winbush, Kansas (Coaches-2)
• Quentin Griffin, Oklahoma (Coaches-2)
• Ja’Mar Toombs, Texas A&M (Coaches-2)
• Reggie White, Oklahoma State (Media-2)

Centers

• Dominic Raiola, Nebraska (Coaches-1; Media-1)
• Randall Cummins, Kansas State (Coaches-1; Media-2)
• Seth McKinney, Texas A&M (Coaches-1; Media-2)
• Ben Bruns, Iowa State (Coaches-2; Media-1)

Guards

• Andre Gurode, Colorado (Coaches-1; Media-2)
• Russ Hochstein, Nebraska (Coaches-2; Media-1)
• Toniu Fonoti, Nebraska (Coaches-2; Media-2)

Tackles

• Leonard Davis, Texas (Coaches-1; Media-1)
• Frank Romero, Oklahoma (Coaches-2; Media-1)
• Milford Stephenson, Kansas State (Coaches-2)
• Scott Kempenich, Oklahoma (Media-2)

Tight ends

• Tracey Wistrom, Nebraska (Coaches-1; Media-1)
• Daniel Graham, Colorado (Coaches-2; Media-2)

Receivers

• Quincy Morgan, Kansas State (Coaches-1; Media-1)
• Robert Ferguson, Texas A&M (Coaches-1; Media-1)
• J. J. Moses, Baylor (Coaches-2; Media-2)
• Antwone Savage, Oklahoma (Coaches-2)
• Tim Baker, Texas Tech (Media-2)

Defensive selections

Defensive linemen

• Mario Fatafehi, Kansas State (Coaches-1; Media-1)
• Casey Hampton, Texas (Coaches-1; Media-1)
• Justin Smith, Missouri (Coaches-1; Media-1)
• Ryan Fisher, Oklahoma (Coaches-1; Media-2)
• Monty Beisel, Kansas State (Coaches-2; Media-1)
• Reggie Hayward, Iowa State (Coaches-2; Media-2)
• Nate Dwyer, Kansas (Coaches-2)
• Pat Mingucci, Missouri (Coaches-2)
• Kris Kocurek, Texas Tech (Coaches-2)
• Chris Johnson, Kansas State (Media-2)
• Kyle Vanden Bosch, Nebraska (Media-2)

Linebackers

• Rocky Calmus, Oklahoma (Coaches-1; Media-1)
• Carlos Polk, Nebraska (Coaches-1; Media-1)
• Jason Glenn, Texas A&M (Coaches-1; Media-1)
• Torrance Marshall, Oklahoma (Coaches-2; Media-1)
• Ben Leber, Kansas State (Coaches-2; Media-2)
• Lawrence Flugence, Texas Tech (Media-2)
• Brian Gamble, Texas A&M (Media-2)
• D. D. Lewis, Texas (Media-2)

Defensive backs

• J. T. Thatcher, Oklahoma (Coaches-1; Media-1)
• Roy Williams, Oklahoma (Coaches-1; Media-1)
• Jerametrius Butler, Kansas State (Coaches-1; Media-2)
• Quentin Jammer, Texas (Coaches-1; Media-2)
• Gary Baxter, Baylor (Coaches-2; Media-1)
• Kevin Curtis, Texas Tech (Coaches-2; Media-1)
• Dyshod Carter, Kansas State (Coaches-2; Media-2)
• Carl Nesmith, Kansas (Coaches-2; Media-2)

Special teams

Kickers

• Jamie Rheem, Kansas State (Coaches-1; Media-2)
• Kris Stockton, Texas (Coaches-2; Media-1)

Punters

• Dan Hadenfeldt, Nebraska (Coaches-1; Media-2)
• Jeff Ferguson, Oklahoma (Coaches-2; Media-1)

All-purpose / Return specialists

• Aaron Lockett, Kansas State (Coaches-1; Media-1)
• J. J. Moses, Iowa State (Coaches-1)
• J. T. Thatcher, Oklahoma (Coaches-2; Media-1)
• Jamaal Fobbs, Oklahoma State (Coaches-2)

Key