The 2000 All-Big Ten Conference football team consists of American football players chosen as All-Big Ten Conference players for the 2000 NCAA Division I-A football season. The conference recognizes two official All-Big Ten selectors: (1) the Big Ten conference coaches selected separate offensive and defensive units and named first- and second-team players (the "Coaches" team); and (2) a panel of sports writers and broadcasters covering the Big Ten also selected offensive and defensive units and named first- and second-team players (the "Media" team).

Offensive selections

Quarterbacks

• Drew Brees, Purdue (Coaches-1; Media-1)
• Antwaan Randle El, Indiana (Coaches-2; Media-2)

Running backs

• Damien Anderson, Northwestern (Coaches-1; Media-1)
• Anthony Thomas, Michigan (Coaches-1; Media-1)
• Michael Bennett, Wisconsin (Coaches-2; Media-2)
• T. J. Duckett, Michigan State (Coaches-2; Media-2)

Receivers

• David Terrell, Michigan (Coaches-1; Media-1)
• Vinny Sutherland, Purdue (Coaches-1; Media-2)
• Ron Johnson, Minnesota (Coaches-2; Media-1)
• Chris Chambers, Wisconsin (Coaches-2)
• Kevin Kasper, Iowa (Media-2)

Centers

• Ben Hamilton, Minnesota (Coaches-1; Media-1)
• LeCharles Bentley, Ohio State (Coaches-2; Media-2)

Guards

• Steve Hutchinson, Michigan (Coaches-1; Media-1)
• Casey Rabach, Wisconsin (Coaches-1; Media-1)
• Bill Ferrario, Wisconsin (Coaches-2; Media-2)
• Ray Redziniak, Illinois (Coaches-2)
• Shaun Mason, Michigan State (Media-2)

Tackles

• Jeff Backus, Michigan (Coaches-1; Media-1)
• Matt Light, Purdue (Coaches-1; Media-1)
• Marques Sullivan, Illinois (Coaches-2; Media-2)
• Kareem McKenzie, Penn State (Coaches-2)
• Leon Brockmeier, Northwestern (Media-2)

Tight ends

• Tim Stratton, Purdue (Coaches-1; Media-1)
• Tony Stewart, Penn State (Coaches-2; Media-2)

Defensive selections

Defensive linemen

• Dwayne Missouri, Northwestern (Coaches-1; Media-1)
• Karon Riley, Minnesota (Coaches-1; Media-1)
• Fred Wakefield, Illinois (Coaches-1; Media-1)
• Wendell Bryant, Wisconsin (Coaches-1; Media-2)
• Akin Ayodele, Purdue (Coaches-2; Media-2)
• Justin Kurpeikis, Penn State (Coaches-2; Media-1)
• Matt Mitrione, Purdue (Coaches-2; Media-2)
• John Schlect, Minnesota (Coaches-2)
• Brent Johnson, Ohio State (Coaches-2)

Linebackers

• Josh Thornhill, Michigan State (Coaches-1; Media-1)
• Joe Cooper, Ohio State (Coaches-1; Media-2)
• Larry Foote, Michigan (Coaches-1; Media-2)
• Nick Greisen, Wisconsin (Media-1)
• Billy Silva, Northwestern (Media-1)
• Napoleon Harris, Northwestern (Coaches-2; Media-2)
• Matt Wilhelm, Ohio State (Coaches-2)
• Sean Hoffman, Minnesota (Coaches-2)
• Justin Smith, Indiana (Coaches-2)

Defensive backs

• Nate Clements, Ohio State (Coaches-1; Media-1)
• Jamar Fletcher, Wisconsin (Coaches-1; Media-1)
• Renaldo Hill, Michigan State (Coaches-1; Media-2)
• James Boyd, Penn State (Coaches-2; Media-1)
• Mike Doss, Ohio State (Coaches-2; Media-1)
• Willie Middlebrooks, Minnesota (Coaches-1)
• Mike Echols, Wisconsin (Coaches-2; Media-2)
• Richard Newsome, Michigan State (Coaches-2)
• Harold Blackmon, Northwestern (Media-2)
• Cedric Henry, Michigan State (Media-2)

Special teams

Kickers

• Dan Stultz, Ohio State (Coaches-1; Media-1)
• Dan Nystrom, Minnesota (Coaches-2)
• Tim Long, Northwestern (Media-2)

Punters

• Kevin Stemke, Wisconsin (Coaches-1; Media-1)
• Preston Gruening, Minnesota (Coaches-2; Media-2)

Key

Bold = selected as a first-team player by both the coaches and media panel

Coaches = selected by Big Ten Conference coaches^[1]

Media = selected by a media panel^[1]