The 2000 Soul Train Music Awards were held on March 4, 2000 at the Shrine Auditorium in Los Angeles, California. The show was hosted by Eric Benét, Lisa "Left Eye" Lopes, Tamia and Shemar Moore.

Special awards

Artist of the Decade for Extraordinary Artistic Achievements – Male

• Prince

Artist of the Decade for Extraordinary Artistic Achievements – Female

• Whitney Houston

Sammy Davis Jr. Award for Entertainer of the Year – Male

• DMX

Sammy Davis Jr. Award for Entertainer of the Year – Female

• Mary J. Blige

Winners and nominees

Winners are in bold text.

R&B/Soul or Rap Album of the Year

• R.Kelly – R.
  • Mary J Blige – Mary
  • DMX – Flesh of My Flesh, Blood of My Blood
  • TLC – Fanmail

Best R&B/Soul Album – Male

• Ginuwine – 100% Ginuwine
  • Eric Benét – A Day in the Life
  • Donell Jones – Where I Wanna Be
  • Brian McKnight – Back at One

Best R&B/Soul Album – Female

• Mary J. Blige – Mary
  • Macy Gray – On How Life Is
  • Whitney Houston – My Love Is Your Love
  • Jennifer Lopez – On the 6

Best R&B/Soul Album – Group, Band, or Duo

• TLC – FanMail
  • Destiny's Child – The Writing's on the Wall
  • K-Ci & JoJo – It's Real
  • Les Nubians – Princesses Nubiennes

Best R&B/Soul Single – Male

• Maxwell – "Fortunate"
  • Ginuwine – "So Anxious"
  • Donell Jones – "U Know What's Up"
  • Brian McKnight – "Back at One"

Best R&B/Soul Single – Female

• Lauryn Hill – "Ex-Factor"
  • Mariah Carey (featuring Jay Z) – "Heartbreaker"
  • Whitney Houston – "My Love Is Your Love"
  • Chanté Moore – "Chanté's Got a Man"

Best R&B/Soul Single – Group, Band, or Duo

• TLC – "No Scrubs"
  • Destiny's Child – "Bills, Bills, Bills"
  • Dru Hill – "Beauty"
  • Ideal – "Get Gone"

The Michael Jackson Award for Best R&B/Soul or Rap Music Video

• Busta Rhymes (featuring Janet Jackson) – "What's It Gonna Be?!"
  • Missy Elliott (featuring Nas, Eve and Lil' Mo) – "Hot Boyz"
  • Q-Tip – "Vivrant Thing"
  • Will Smith – "Will 2K"

Best R&B/Soul or Rap New Artist

• Juvenile
  • Eve
  • Ideal
  • Angie Stone

Best Gospel Album

• Dottie Peoples – God Can & God Will
  • Dorothy Norwood – The Lord is a Wonder
  • Richard Smallwood – Healing: Live in Detroit
  • Vickie Winans – Live in Detroit, Vol. 2

Presenters