Brook is a surname. Notable people with the surname include:

• Abraham Brook (flourished 1789), English physicist and bookseller
• Andrew Brook (born 1943), Canadian philosopher
• Basil Brook (1576 – c. 1646), English ironmaster, see Basil Brooke (metallurgist)
• Benjamin Brook (1776–1848), English nonconformist minister and religious historian
• Charles Brook (philanthropist) (1814–1872), English philanthropist
• Claudio Brook (1927–1995), Mexican actor
• Clive Brook (1887–1974), English actor
• David Brook (disambiguation)
• Eric Brook (1907–1965), English footballer
• Faith Brook (1922–2012), English actress
• George Brook (cricketer) (1888–1966), English cricketer
• George Brook (zoologist) [species], British zoologist
• Gina Knee Brook (1898–1982), American painter
• Harry Brook (born 1999). English cricketer
• Helen Brook (1907–1997), British family planning adviser
• Holly Brook (born 1986), AKA Skylar Grey, American singer and songwriter
• Jayne Brook (born 1962), American actress
• Kelly Brook (born 1979), English model
• Michael Brook (born 1951), Canadian guitarist, inventor, producer, and film music composer
• Norman Brook, 1st Baron Normanbrook (1902–1967), British civil servant.
• Peter Brook (1925–2022), English theatre and film director and innovator
• Rhidian Brook (born 1964), British novelist, screenwriter and broadcaster
• Richard Brook (chief executive), English executive
• Timothy Brook (born 1951), Canadian historian
• Tom Brook (born 1953), BBC World presenter
• William Broke or Brook, English 16th-century college and university head
• Yaron Brook (born 1961), Israeli-American entrepreneur and writer