CHKB
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3FEG, 2IG7, 3LQ3
Identifiers
Aliases	CHKB, CHETK, CHKL, CK, CKB, CKEKB, EK, EKB, MDCMC, choline kinase beta
External IDs	OMIM: 612395 MGI: 1328313 HomoloGene: 88718 GeneCards: CHKB
Gene location (Human) Chr. Chromosome 22 (human)[1] Band 22q13.33 Start 50,578,959 bp[1] End 50,601,455 bp[1]
Gene location (Mouse) Chr. Chromosome 15 (mouse)[2] Band 15|15 E3 Start 89,310,563 bp[2] End 89,314,111 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in pituitary gland anterior pituitary spleen right lobe of thyroid gland left lobe of thyroid gland right uterine tube gastric mucosa prostate minor salivary gland lymph node Top expressed in islet of Langerhans bone marrow colon duodenum jejunum spleen ileum zone of skin cerebellar cortex spermatocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding ATP binding kinase activity choline kinase activity ethanolamine kinase activity Cellular component cytosol Biological process phosphatidylcholine biosynthetic process phosphatidylethanolamine biosynthetic process phosphorylation lipid metabolism phospholipid biosynthetic process glycerophospholipid biosynthetic process CDP-choline pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1120 12651 Ensembl ENSG00000100288 ENSMUSG00000022617 UniProt Q9Y259 O55229 RefSeq (mRNA) NM_152253 NM_005198 NM_007692 RefSeq (protein) NP_005189 NP_031718 Location (UCSC) Chr 22: 50.58 – 50.6 Mb Chr 15: 89.31 – 89.31 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Choline kinase beta (CK), also known as Ethanolamine kinase (EK), Choline kinase-like protein , choline/ethanolamine kinase beta (CKEKB), or Choline/ethanolamine kinase is a protein encoded by the CHKB gene.^[5][6] This gene is found on chromosome 22 in humans. The encoded protein plays a key role in phospholipid biosynthesis. Choline kinase (CK) and ethanolamine kinase (EK) catalyzes the first step in phosphatidylethanolamine biosynthesis. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.^[7][8][9]

Structure

The CHKB gene is located on the q arm of chromosome 22 at position 13.3 and it spans 4,041 base pairs.^[7] The CHKB gene produces a 7 kDa protein composed of 60 amino acids.^[10][11] The structure of the protein has been found to be a homodimer, and forms a heterodimer with the CHKA protein.^[9][8] It has also been found to contain multiple highly conserved domains, such as a Brenner phosphotransferase consensus sequence essential in catalytic function.^[12]

Function

The CHKB gene encodes for a key protein in phospholipid biosynthesis. The choline kinase (CK) and ethanolamine kinase (EK) proteins, which are coded by the CHKB gene, catalyze the phosphorylation of choline/ethanolamine in vitro to phosphocholine/phosphoethanolamine. The catalysis is controlled by ATP in the presence of magnesium and ADP, and commits choline to the enzymatic pathway for biosynthesis of phosphatidylcholine. This is the first step in the biosynthesis of phosphocholine/phosphoethanolamine in all animal cells, and is done by the Kennedy pathway. The highly purified choline kinases from mammalian sources and their recombinant gene products have been shown to have ethanolamine kinase activity as well, indicating that both activities reside on the same protein. However, it has been shown that the protein has higher activity with ethanolamine and may not significantly contribute to in vivo phosphatidylcholine biosynthesis. The choline kinase-like protein encoded by CHKB belongs to the choline/ethanolamine kinase family; however, its exact function is not known. At least two transcript variants encoding two different isoforms have been found for this gene, and one of the transcripts is bicistronic.^{[7][8][9][13][14]}

Clinical significance

Mutations in CHKB have been found to result in mitochondrial deficiencies and associated disorders. Knockdown of the gene has been known to result in decreased choline kinase and phosphatidylcholine activity. This impairment in activity may lead to a modified composition of the phospholipid composition in the mitochondrial membrane resulting in major disorders in the function and structure of the mitochondria. Major disorders include as Megaconial Congenital Muscular Dystrophy (MDCMC), and Narcolepsy.^[7][15]

Megaconial Congenital Muscular Dystrophy (MDCMC)

CHKB mutations have been majorly associated with Megaconial Congenital Muscular Dystrophy (MDCMC). Megaconial Congenital Muscular Dystrophy (MDCMC) is an autosomal recessive congenital muscular dystrophy characterized by muscle biopsy results displaying an enlarged mitochondria which are common in the periphery of the fibers but scarce around the center.^[16]

Common clinical manifestations of MDCMC include:^[15][17]

• early-onset hypotonia
• muscle wasting
• mildly elevated serum creatine kinase (CK) levels
• severe intellectual disability without brain structural abnormalities
• Gower's sign
• mental retardation
• fatal cardiomyopathy

Symptoms such as neurogenic atrophy, enlarged mitochondria in the periphery of the fibers, and complex I deficiency were shown in a Spanish patient with a homozygous mutation of c.810T>A.^[15] Another patient with a homozygous mutation (p.E292X) in the CHKB gene exhibited rhythmic jerkings of arms, which were characterized as muscle spasms.^[14] Finally, a patient with a homozygous c.810T>A showed signs of Gower's sign, hypotonia, and proximal muscle weakness.^[18]

Narcolepsy

Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.^[19]

Interactions

CHKB has been shown to have Protein-protein interactions with the following.^[20][8]

• ALB
• Human serum albumin