DAOY is a widely used human medulloblastoma cell line. It has epithelial morphology and was obtained from a 4-year-old Caucasian boy in 1985.[1] It can be obtained from the American Type Culture Collection (ATCC) where it is known by the catalog number HTB-186.[2] Its proteome and phenotype have been investigated in many publications.[3][4][5]

DAOY is most commonly grown in Eagle's minimal essential medium with 10% FBS and antibiotics. It is incubated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.

References

  1. ^ Jacobsen PF, Jenkyn DJ, Papadimitriou JM (Sep 1985). "Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice". J Neuropathol Exp Neurol. 44 (5): 472–485. doi:10.1097/00005072-198509000-00003. PMID 2993532. S2CID 8681884.
  2. ^ "HTB-186". ATCC.
  3. ^ Martelli C, et al. (Sep 2016). "Top-down proteomic characterization of DAOY medulloblastoma tumor cell line". EuPA Open Proteomics. 12: 13–21. doi:10.1016/j.euprot.2016.03.015. PMC 5988510. PMID 29900115.
  4. ^ Srivastava VK, Nalbantoglu J (Oct 2008). "Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer stem-like phenotype". Cytometry A. 73 (10): 940–948. doi:10.1002/cyto.a.20633. PMID 18773455.

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