Orthography

Full stop or "period", a sentence terminator
Dot (diacritic), a mark above or below a character (e.g. ȧ, ạ, İ, Ċ, ċ, etc.), usually to indicate sound mutation
Interpunct (·) also known as an "interpoint", "middle dot", "middot", "centered dot" or "centred dot", a punctuation mark

Mathematics

"Dot as decimal point", the form of decimal separator generally used in anglophone countries, e.g., $24.99
Dot operator, a notation used to signify multiplication
Dot product, algebraic operation returning a single number from two equal-length sequences

The Dot (Toyism), a work of art in Emmen, the Netherlands
"Dot" (song), a single by the Los Angeles pop punk band ALL
"Dot", a song by Destiny's Child featured on the Charlie's Angels soundtrack
Dot, A Drama in Three Acts, an 1859 stage adaption of the book The Cricket on the Hearth by Charles Dickens
The Dot (book), a 2003 children's picture book
The Dot (computer), a portable computer
Dot., a 2013 children's book adapted into a 2016 TV series
Dot (film), a 2010 British animated short film
[Dot, British radio series written by Ed Harris, first released in 2015
The Dot (restaurant), a fictional café in Degrassi: The Next Generation
WTTK, A local television station broadcasting as "The Dot"

Dot Cycle and Motor Manufacturing Company, a British motorcycle manufacturing company established in 1903
Dot Foods, the largest foodservice redistribution company in the United States
Dot Records, an American company

Dot (dit), an element in Morse code
Dot (Unix)
DOT (graph description language), in computation, the plain-text format for describing graphs used by Graphviz software
".", the DNS root zone, the root domain of the Domain Name System
DoT, DNS over TLS
DOT code, an alphanumeric character sequence for purposes of tire identification
Damage over time, in computer and video games
Designated Order Turnaround or SuperDot, an electronic system used by NYSE to route market orders
Diffuse optical imaging, also known as diffuse optical tomography, a medical imaging technique
Directly observed treatment, a way to administer medicine
Dissolved oxygen tension (DOT)
Dutch Open Telescope
The Dot, an Office Assistant, one of several interactive animated characters in Microsoft Office
Amazon Echo Dot, also called the Dot, part of a brand of smart speakers developed by Amazon

Dot (given name), a list of people and fictional characters
Pedro Dot (1885–1976), Spanish rose breeder
Admiral Dot, stage name of Leopold S. Kahn (1859 or 1863–1918), a dwarf performer for P. T. Barnum
Dot, one of the mascots of PBS Kids since 1999