Egg drop syndrome '76 (EDS '76) is a viral disease that affects birds, notably chickens, ducks, geese and swans. It is characterised by a sudden drop in production of eggs as well as its eggshell quality in apparent healthy laying birds.[1]
Previously, this disease was commonly named as merely "egg drop syndrome"; however, it is now recommended that full name, egg drop syndrome '76 (EDS '76), should be used to distinguish the name discrepancy from the recently identified disease in ducks caused by a flavivirus, which is confusingly called "egg drop syndrome in ducks" or "duck egg drop syndrome".
Incidence
EDS '76 was first identified in Netherlands in 1974[2] and the virus was first isolated in Northern Ireland in 1976.[3] It became a problem in European countries such as Ireland, the Netherlands, France, and the United Kingdom. Other places which have had outbreaks include India,[4] Bangladesh,[5] South America,[6] Indonesia,[7] Japan,[citation needed] and Africa.[8] Since then, EDS '76 has been recognised as a global threat for the breeding and laying companies.
The other name of this virus may include duck adenovirus 1 (DAdV-1), EDS '76 virus (EDSV) and adenovirus 127 (AV-127).[9]
Species affected
The natural host of Duck atadenovirus A are wild and/or domestic waterfowls such as ducks and geese. However, the virus can also infect other bird species through contaminated vaccines,[3] and contaminated drinking water by droppings.[9]
Moreover, the virus become apparent and more susceptible in chickens of all ages and breeds, especially broilers and brown egg layers. The virus also causes symptoms in Japanese quails (Coturnix coturnix japonica)[citation needed] and turkeys.[10]
Transmission
Duck atadenovirus A can be transmitted vertically (from hens to chicks). The virus is often latent until the chicks reach maturity. Thereafter, the matured chickens begin to excrete virus and transmit through the eggs and droppings.
The virus is also transmitted horizontally between chickens. Mainly, it is seen in commercial egg layers whereby contaminated egg trays are often reused. The virus can survive both inside the eggs and on the eggshell. The virus can migrate from the eggshell to the tray and back to the other eggs placed in the same tray. Contaminated water supply with droppings and litters of the natural host contributes to the horizontal spread as well.[1]
The first sign of EDS '76 is usually the loss of colour pigment in the eggs, followed by the production of thin-shelled, soft-shelled and even shell-less eggs. The thin-shelled and shell-less eggs are so fragile and thus may get eaten by the chickens and get disregarded as litters. The shells may also be rough or chalky. Watery egg whites and a reduction in egg size may also happen as well.[12][13]
The affected chicken flocks show a failure to reach a peak egg production. The fall in egg production can be up to 40%. The affected chickens may show transient diarrhoea, anaemia, and loss of appetite. Increase in mortality is not observed.[9][14]
Gross Lesions EDS '76. A; normal hens' reproductive system B; inactive ovaries and shrunken oviducts
Lesions
The lesions are confined to female reproductive tracts. These may include inactive ovaries and shrunken oviducts, swelling and white exudate in the shell gland. Spleen enlargement may also be observed.[9]
Diagnosis and detection
EDS '76 can largely be distinguished from other poultry diseases, such as Avian Influenza, Infectious Bronchitis and Newcastle Disease, by the clinical findings alone. However, proper laboratory testing is still needed to eliminate doubts for a definitive diagnosis.[1]
Serological testing can determine if chicken have antibodies against EDS '76, which would indicate that they have been infected. This is only possible for non-vaccinated chickens because vaccination or infection both cause antibodies to be present. The common tests of choice include Haemaglutination-Inhibition, Enzyme-linked Immunosorbent Assay and Serum Neutralisation.[15][16]
The use of sentinel birds (non-vaccinated susceptible birds) in the flocks is also useful for early detection and tested for haemagglutination. It is common practice for poultry viruses' surveillance.[17]
There is no treatment for EDS '76, but there are ways in which chickens can be prevented from being infected by EDS '76.
Hygiene:
Cleaning all areas, such as breeding and laying areas, and equipment may mitigate the risk of getting EDS '76. Shared egg trays have to be cleaned and disinfected prior to use. Healthy and uninfected flocks should be kept separated from infected birds and from the virus' natural host. Potentially contaminated water should also be chlorinated.[1]
Vaccination:
An inactivated vaccine with oil adjuvant can be administered and has proven to be reasonably successful to prevent the clinical signs of the disease. This vaccine is given before hens start to lay eggs, usually between 14 and 20 weeks of age. Several vaccines are available: Izovac EDS, Nobilis EDS, AVIVAC-EDS-76 and other EDS '76 available vaccines.
^Mohanty GC, Pradhan HK, Verma KC, et al. (1984). "Egg Drop Syndrome (EDS -'76) in India: Seroprevalence of EDS -'76 Virus Infections in Poultry Flocks". Indian Journal of Poultry Science. 19: 15–18.
^Biswas PK, Barua H, Uddin GM, Biswas D, Ahad A, Debnath NC (January 2009). "Serosurvey of five viruses in chickens on smallholdings in Bangladesh". Prev Vet Med. 88 (1): 67–71. doi:10.1016/j.prevetmed.2008.06.018. PMID18723236.
^Ezema WS, Nwanta J, Aka L, et al. (2010). "Egg-Drop Syndrome 76 in different bird species in Nigeria - A review of the epidemiology, economic losses, challenges and prospect for management and control". World's Poultry Science Journal. 66 (1): 115–122. doi:10.1017/S0043933910000115. S2CID86205847.
^Van Eck JH, Vertommen M, Van den HP, et al. (1977). "Recent studies on the role of adenovirus in the problem of dropped egg production and production of soft shelled and shell-less eggs". In Proc. Conf. On Avian Adenoviruses and Infectious Bronchitis: 42–44.
^Van Eck JH, Elanbass L, Wensvoort P, et al. (1978). "Histopathological changes in the oviduct of hens producing shell-less eggs associated with precipitins to adenovirus". Avian Pathology. 7 (2): 279–287. doi:10.1080/03079457808418279. PMID18770379.
^Darbyshire JH, Peters RW (2007). "Studies on EDS-76 virus infection in laying chickens". Avian Pathology. 9 (3): 277–290. doi:10.1080/03079458008418413. PMID18770267.
^Volkova VA, Chvala IA, Mudrak NS (2016). "Evaluation of efficiency of ELISA for detection of antibodies to EDS-76 virus". Russian Veterinary Journal. Productive Animals. 2: 21–24.
