State Road 326
SR 326 in red, segments of CR 326 in blue
Route information
Maintained by FDOT
Length	11.577 mi[1] (18.631 km)
Major junctions
West end	I-75 near Zuber
Major intersections	US 301 / US 441 near Zuber
East end	SR 40 near Silver Springs
Location
Country	United States
State	Florida
Counties	Marion
Highway system
Florida State Highway System Interstate US State Former Pre‑1945 Toll Scenic
← SR 320 → SR 327

State Road 326 (SR 326) is an east–west route in Marion County, Florida, in and around Ocala. The western terminus is near Interstate 75's (I-75) exit 358, and its eastern end is at SR 40 east of Silver Springs. The highway acts as a bypass around Ocala, taking traffic to SR 40 on the edge of the Ocala National Forest.

West of I-75, the road continues as County Road 326 (CR 326) in Marion and Levy counties to the Waccasassa River near Gulf Hammock with another segment leading to the Lower Suwannee National Wildlife Refuge adjacent to the Gulf of Mexico.

Major intersections

County	Location	mi[1][2]	km	Destinations	Notes
Levy	Williams Camp	0.000	0.000	dead end at Waccasassa River
	Gulf Hammock	3.864	6.219	US 19 / US 98 (SR 55)
	​	7.1[3]	11.4	CR 343 north – Henry Beck Park
	​	13.3[3]	21.4	CR 337
	​	16.7[3]	26.9	SR 121
	​	22.102	35.570	US 41 north (SR 45)	west end of US 41 overlap
	​	22.362	35.988	US 41 south (SR 45)	east end of US 41 overlap; west end of CR 323 overlap
	Morriston	22.7[3]	36.5	CR 323 north (Southeast 32nd Place)	east end of CR 323 overlap
Marion	Blitchton	29.1[3]	46.8	US 27 (SR 500)
	​	31.8[3]	51.2	CR 225
	​	35.6[3]	57.3	CR 225A
	​	37.616	60.537	west end of state maintenance
	​	38.11	61.33	I-75 (SR 93) – Lake City, Tampa	I-75 exit 358
	Zuber	38.859	62.537	CR 25A – Fire College, Speedway
	​	40.733	65.553	US 301 / US 441 (SR 25 / SR 200) – Gainesville, Ocala
	Oak	43.049	69.281	CR 200A
	​	47.171	75.914	CR 35 (Northeast 58th Avenue)
	​	49.193	79.168	SR 40 – Ormond Beach, Silver Springs
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus       Route transition