Henry Lee may refer to:

People

• Sir Henry Lee of Ditchley (1533–1611), Master of the Ordnance and Queen's Champion under Elizabeth I of England, MP for Buckinghamshire
• Henry Lee (Canterbury MP) (c. 1657–1734), MP for Canterbury
• Capt. Henry Lee I (1691–1747), of Lee Hall, Westmoreland County Virginia; Virginian colonist, grandfather of Henry Lee III
• Major General Henry Lee II (1730–1787), of Leesylvania, father of Henry "Light-Horse Harry" Lee III
• Major General Henry Lee III (1756–1818), nicknamed "Light-Horse Harry", Virginia governor and Congressman as well as early American officer
• Henry Lee IV (1787–1837), also known as "Black Horse Harry" Lee, half-brother of Robert E. Lee and son of Henry Lee III
• Henry Lee (economist) (1782–1867), proponent of free trade and candidate for Vice President of the United States in 1832
• Henry Lee (naturalist) (1826?–1888), English aquarium director and author
• Henry Lee (Australian politician) (1856–1927), Australian politician
• Henry A. G. Lee (c. 1818–1851), newspaper editor, politician, militia officer in Oregon, United States
• Henry B. Lee (1781–1817), U.S. Representative from New York
• Henry David Lee (1849–1928), American businessman
• Henry W. Lee (socialist) (1865–1932), British socialist
• Henry Lee (forensic scientist) (born 1938), Chinese-American forensic scientist
• Henry Wesley Lee, American child actor who played "Spike" in the Our Gang shorts
• Henry Lee (Southampton MP) (1817–1904), British Member of Parliament for Southampton, 1880–1885
• Henry Lee Junior (born 1958), Hong Kong celebrity and racing driver
• Henry Lee (actor) (born 1949), Hong Kong TVB actor
• Henry W. Lee (bishop) (1815–1874), Episcopal bishop in America
• Henry Lee (cyclist) (1887–?), British Olympic cyclist
• Henry Austin Lee (1847–1918), British diplomat, governor and landowner
• Henry Pelham Lee (1877–1953), English engine pioneer
• Henry Lee (surgeon), British surgeon, pathologist and syphilologist
• Henry Lee Lucas (1936–2001), American criminal, convicted of murder and once listed as America's most prolific serial killer

Music

• "Henry Lee", an alternate title of "Young Hunting", a traditional murder ballad originating in Scotland.