Alpha trans-inducing protein (Alpha-TIF)
Structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16.[1]
Identifiers
SymbolAlpha_TIF
PfamPF02232
InterProIPR003174
SMARTSM00814
SCOP216vp / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary
PDB16vp​

Vmw65, also known as VP16 or α-TIF (Trans Inducing Factor)[2] is a trans-acting protein that forms a complex with the host transcription factors Oct-1 and HCF to induce immediate early gene transcription in the herpes simplex viruses.[3][4]

VP16 is a strong transactivator[5] and is often used in Y2H systems as the activation domain of the system.[6]

References

  1. ^ Liu Y, Gong W, Huang CC, Herr W, Cheng X (July 1999). "Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16". Genes Dev. 13 (13): 1692–703. doi:10.1101/gad.13.13.1692. PMC 316849. PMID 10398682.
  2. ^ Entry for Vmw65 on Cure hunter
  3. ^ O'Reilly D, Hanscombe O, O'Hare P (May 1997). "A single serine residue at position 375 of VP16 is critical for complex assembly with Oct-1 and HCF and is a target of phosphorylation by casein kinase II". EMBO J. 16 (9): 2420–30. doi:10.1093/emboj/16.9.2420. PMC 1169842. PMID 9171355.
  4. ^ Wysocka J, Herr W (June 2003). "The herpes simplex virus VP16-induced complex: the makings of a regulatory switch". Trends Biochem. Sci. 28 (6): 294–304. doi:10.1016/S0968-0004(03)00088-4. PMID 12826401.
  5. ^ Hirai, Hiroyuki; Tani, Tetsuya; Kikyo, Nobuaki (2010). "Structure and functions of powerful transactivators: VP16, MyoD and FoxA". The International Journal of Developmental Biology. 54 (11–12): 1589–1596. doi:10.1387/ijdb.103194hh. PMC 3419751. PMID 21404180.
  6. ^ Stynen, B.; Tournu, H.; Tavernier, J.; Van Dijck, P. (11 June 2012). "Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System". Microbiology and Molecular Biology Reviews. 76 (2): 331–382. doi:10.1128/MMBR.05021-11. PMC 3372256. PMID 22688816.


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