The following is a list of players, both past and current, who appeared at least in one game for the Houston Astros National League franchise (1965–2012) and current American League franchise (2013–present), also known previously as the Houston Colt .45's (1962–1964).

Players in bold are members of the National Baseball Hall of Fame.

Players in italics are members of the Houston Astros Hall of Fame.

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

Y

Z