Microscopic image of the keratin cytoskeleton of a HUVEC cell.

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis).[1] They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth. HUVECs were first isolated and cultured in vitro in the 1970s by Jaffe and others. [2] HUVECs can be easily made to proliferate in a laboratory setting. Like human umbilical artery endothelial cells they exhibit a cobblestone phenotype when lining vessel walls.

Inhibition of the sirtuin protein sirtuin 1 (SIRT1) in HUVECs has been shown to induce cellular senescence.[3] Conversely, overexpression of SIRT1 in HUVECs has been shown to inhibit cellular senescence.[4] The polyphenol quercetin has been found to be an effective senolytic for inducing the death of senescent HUVECs.[4]

References

  1. ^ Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB (2006). "Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis". Stem Cell Rev. 2 (2): 93–102. doi:10.1007/s12015-006-0015-x. PMID 17237547. S2CID 31350461.
  2. ^ Jiménez, N., Krouwer, V. & Post, J. A new, rapid and reproducible method to obtain high quality endothelium in vitro. Cytotechnology 65, 1-14 (2012).
  3. ^ Wang Y, Liang Y, Vanhoutte PM (2011). "SIRT1 and AMPK in regulating mammalian senescence: a critical review and a working model". FEBS Letters. 585 (7): 986–994. doi:10.1016/j.febslet.2010.11.047. hdl:10722/142456. PMID 21130086. S2CID 185902.
  4. ^ a b Kim E, Kim J (2019). "Senotherapeutics: emerging strategy for healthy aging and age-related disease". BMB Reports. 52 (1): 47–55. doi:10.5483/BMBRep.2019.52.1.293. PMC 6386227. PMID 30526770.

External links

Category