Indocyanine green angiography
Choroidal blood flow revealed with indocyanine green angiography
ICD-9-CM95.11

Indocyanine green angiography (ICGA) is a diagnostic procedure used to examine choroidal blood flow and associated pathology. Indocyanine green (ICG) is a water soluble cyanine dye which shows fluorescence in near-infrared (790–805 nm) range, with peak spectral absorption of 800-810 nm in blood.[1][2] The near infrared light used in ICGA penetrates ocular pigments such as melanin and xanthophyll, as well as exudates and thin layers of sub-retinal vessels.[3] Age-related macular degeneration is the third main cause of blindness worldwide, and it is the leading cause of blindness in industrialized countries.[4] Indocyanine green angiography is widely used to study choroidal neovascularization in patients with exudative age-related macular degeneration.[5] In nonexudative AMD, ICGA is used in classification of drusen and associated subretinal deposits.[5]

Indications

Indications for indocyanine green angiography include:

Procedure

Fundus camera-based indocyanine green angiography techniques and scanning laser ophthalmoscope-based indocyanine green angiography techniques are there.[9] The concentration of indocyanine green dye may vary according to instrument used. For fundus cameras, 25 ml ICG dissolved in 5 ml solvent is used, it may be increased to 50 ml in patients with poorly dilated pupil and high pigmentation.[6] In case of iodine allergy, instead of ICG, iodine-free dye Infracyanine green should be used.[10]

To perform test, pupil should be dilated. The dye is injected through the antecubital vein as bolus.[11] Images are taken in several second intervals until the retinal and choroidal circulations are maximally hyperfluorescent.[9] Then for first few minutes, take photos at approximately 30 to 60 second intervals. Pictures taken are classified under three phases:[12]

  1. Early phase at 60 seconds: large choroidal arteries and veins are highlighted in this phase.[13]
  2. Mid phase at 5–15 minutes: in this phase choroidal vasculature become less distinct and more diffuse, and hyperfluorescent lesions appear bright against the fading background.[13]
  3. Late phase at 15–30 minutes: in this phase hyperfluorescent lesions appear bright against the dark background.[13] The choroidal neovascularization are best detected in this phase.[13]

Advantages over fluorescein angiography

Indocyanine green angiography has many advantages over commonly used fundus fluorescein angiography (FFA). Because of its protein-binding properties, its leakage from choriocapillaries is less and thus it will remain longer in choroidal vessels compared to fluorescein dye.[1] Choroidal neovascularization is better visualized by ICGA, than fluorescein angiography.[5] The patient toleration is also better compared to FFA.[11]

History

Physical and physiological properties of indocyanine green dye were first described by Fox and Wood, in 1960.[14] Indocyanine green angiography was developed by Kodak Research Laboratories for determining cardiac output. In 1968, Kogure et al. performed intra-arterial choroidal absorption angiography using indocyanine green dye in monkeys.[15] In the year 1969, using ICGA, Kogure and Choromokos studied cerebral circulation in a dog. In 1971, Hochhimer replaced color film with black and white infrared film. First human ICG angiogram was of carotid artery. First intravenous ICGA in human eye was performed by Flower and Hochheimer in 1972.[11][6] In 1986 Hayashi et al. used infrared-sensitive video camera to perform ICGA.[9] In the year 1992, Guyer et al. introduced the use of high resolution (1024 × 1024) digital imaging system coupled with infrared video cameras to produce better high resolution images.[16]

See also

References

  1. ^ a b c "Indocyanine Green Angiography". www.aao.org.
  2. ^ a b c d e f Khurana, AK (31 August 2015). "Ocular therapeutics". Comprehensive ophthalmology (6th ed.). Jaypee, The Health Sciences Publisher. p. 460. ISBN 978-93-5152-657-5.
  3. ^ a b c d e John F, Salmon (2020). "Uveitis". Kanski's clinical ophthalmology : a systematic approach (9th ed.). Elsevier. p. 431. ISBN 978-0-7020-7711-1.
  4. ^ "Priority eye diseases". www.who.int. Archived from the original on March 22, 2006.
  5. ^ a b c d "Diagnostic usefulness of indocyanine green angiography (ICGA) in age-related macular degeneration (AMD)". www.amdbook.org.
  6. ^ a b c d e f g h Andrew P., Schachat (31 May 2017). "Clinical applications of diagnostic indocyanine green angiography". Ryan's retina Vol 1 (6th ed.). pp. 46–76. ISBN 978-0-323-40197-5.
  7. ^ Creig S, Hoyt; David, Taylor (January 2012). Pediatric ophthalmology and strabismus (4th ed.). Saunders/Elsevier. p. 524. ISBN 9780702046919.
  8. ^ a b Albert & Jakobiec's principles and practice of ophthalmology. Albert, Daniel M., Miller, Joan W. (Joan Whitten), Azar, Dimitri T. (3rd ed.). Philadelphia: Saunders/Elsevier. 2008. ISBN 978-1-336-22997-6. OCLC 489078723.((cite book)): CS1 maint: others (link)
  9. ^ a b c Themes, U. F. O. (10 July 2016). "Indocyanine Green Angiography". Ento Key.
  10. ^ Dithmar, S. (2008). Fluorescence angiography in ophthalmology. Frank G. Holz. Heidelberg: Springer Medizin Verlag. p. 3. ISBN 978-3-540-79401-1. OCLC 288578300.
  11. ^ a b c "History, Clinical Examinations and Techniques of Examination". Comprehensive manual of ophthalmology. Jaypee Brothers. 15 June 2010. p. 122. ISBN 978-93-5025-175-1.
  12. ^ PK, Mukherjee (2016). Clinical examination in ophthalmology (2nd ed.). Elsevier. ISBN 978-81-312-4463-0.
  13. ^ a b c d Principles of ocular imaging : a comprehensive guide for the eye specialist. Daniel Gologorsky, Richard B. Rosen. Thorofare, NJ. 2021. p. 93. ISBN 978-1-63091-600-8. OCLC 1259320518.((cite book)): CS1 maint: location missing publisher (link) CS1 maint: others (link)
  14. ^ Owens, S L (March 1996). "Indocyanine green angiography". The British Journal of Ophthalmology. 80 (3): 263–266. doi:10.1136/bjo.80.3.263. ISSN 0007-1161. PMC 505439. PMID 8703866.
  15. ^ Earl A., Choromokos; Noble J., David. "The Beginnings of Indocyanine Green Angiography" (PDF). Journal of Ophthalmic Photography. 16: 70.
  16. ^ Themes, U. F. O. (21 December 2015). "Clinical Applications of Diagnostic Indocyanine Green Angiography". Radiology Key.
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