Japanese rhinoceros beetle
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Coleoptera
Family: Scarabaeidae
Genus: Allomyrina
Species:
A. dichotoma
Binomial name
Allomyrina dichotoma
(Linnaeus, 1771) [1]
Synonyms
  • Trypoxylus dichotomus
  • Allomyrina dichotomus

Allomyrina dichotoma, also known as Japanese rhinoceros beetle, Japanese horned beetle, or kabutomushi (カブトムシ, lit. 'Kabuto insect'), is a species of rhinoceros beetle. They are commonly found in continental Asia in countries such as China, the Korean peninsula, and Japan.[2] In these areas, this species of beetle are often found in broad-leaved forests with tropical or sub-tropical climates. They can also be found in mountainous areas.[2] This beetle is well known for the prominent cephalic horn found on males. This horn can grow to be up to one-third of the beetle's body length and the tip of the horn branches into a "V" shape.[3] Male Japanese rhinoceros beetles will use this horn to fight other males for territory and access to female mating partners. Upon contact, males will attempt to flip each other onto their backs or off of their feeding tree.[3] In response to selective pressures, smaller male A. dichotoma have adapted a "sneak-like behavior". These smaller beetles will attempt to avoid physical confrontation with larger males and try to mate with females.[3]

The large size of the Japanese rhinoceros beetle makes it a useful model organism.[4] The prominent horn on the males makes this species a popular model organism for the study of sexual dimorphic traits.[4] This beetle also has a long history of use in traditional Chinese medicine, which inspired research studies to corroborate its use.[2] To the surprise of many researchers, compounds found in the extracts of A. dichotoma larvae have proven to exhibit anti-obesity effects as well as antibiotic properties.[5][6]

List of subspecies

[7] [8]

Description

A. dichotoma male from Taiwan

Male A. dichotoma have a distinct sexually dimorphic horn protruding from the base of its head which can reach a length of up to one-third its body length.[3] These beetles have a dark brown and red appearance. Their bodies can appear to be black without direct light.[4] On average, males tend to measure between 40 and 80 mm, while females are typically smaller, growing between 35 and 60 mm long. The length of the male A. dichotoma elytra has been recorded to be between 19 and 33 mm and the male horn can range between 7 and 32 mm. As the horn is a sexually dimorphic trait, only male Japanese rhinoceros beetles will grow one.[9] This cephalic horn is typically somewhat thin and "pitchfork shaped".[10] This appendage acts as a lever arm and is commonly used as a tool to fight other males for access to territory and females.[11] Despite the large size of the cephalic horn, male Japanese rhinoceros beetles are still capable of flight; male and females have been reported to fly at similar average speeds.[12] Males with horns that are proportionately large compared to their body size possess larger wings to compensate.[12]

Geographic range

A. dichotoma can be found widely distributed throughout Asia, including China, Japan, Vietnam, Myanmar, Laos, India, Thailand, and the Korean Peninsula. This beetle species prefers to live in broad-leaved forests with tropical or sub-tropical climates. They can also be found often in mountainous environments.[2] Across populations and regions, male beetles can vary greatly in size and horn performance, and it is suggested that differences are due to relative intensities of selection.[13]

Habitat

Bark-carving behavior

Adult Japanese rhinoceros beetles un-burrow from the Earth during the summer months between June and August.[14] They prefer to congregate on wounded tree trunks. Quercus acustissima, Quercus serrata, and Quercus mongolica grosseserrata are the most common trees they choose.[14] A tree wound is caused by boring insects which break through the exterior of the tree and feed on the nutrient rich sap on the interior. Adult A. dichotoma take advantage of the easily accessible food and consume the exposed tree sap. A subspecies of A. dichotoma known as Trypoxylus dichotomus septentrionalis exhibits bark-carving behavior.[14] This variety of Japanese rhinoceros beetle does not require other insects to breach the tough arboreal exterior to access sap. Notably, these beetles conduct this behavior on Fraxinus griffithii trees, which have a thinner bark than the aforementioned species; this thinner exterior is considerably easier to cut through.[14] These beetles cut into the tree by using their clypeus as a chisel. They hold on tightly to the tree and move their head back and forth to make a cut into the bark.[14] For a short time, sap flows out of the newly made wound, and the Japanese rhinoceros beetle can feed. After a few minutes, the sap stops flowing, so the beetle begins to carve again.

Life history

Third instar larva (last step before metamorphosis)

Female A. dichotoma beetles oviposit by scattering their eggs in the humus portion of soil during July and September. The larvae feed on the humus, develop into the third instar phase and pupate during June-July of the following year. Adult beetles emerge from the soil in the few months after pupating. It takes A. dichotoma beetles 1 year to develop into adults after being laid as eggs. [15]

Larvae behavior

Larval aggregation in A. dichotoma is driven by chemical cues.[16] The larvae in this species burrow into the dirt, so chemical and acoustic cues are more relevant than visual cues. Studies have shown that chemical cues are necessary for larvae gathering. Larvae with nonfunctioning chemosensory organs could not aggregate, so chemical cues are likely an important signal guiding larval aggregation.[16] Notably, 1st-instar larvae do not aggregate with other larvae because they demonstrate cannibalistic tendencies at this stage. 2nd- and 3rd-instar larvae are not cannibalistic, so they aggregate normally. These larvae also do not discriminate based on kinship when they group. They will group at conspecific larvae but do not demonstrate preferences based on shared genetic similarities. Mature larvae have been shown to construct pupal cells close to groups of larvae that are already living in the soil. These larvae recognize chemicals produced by other larvae and use these signals to determine where they will make their pupation site.[16] One main benefit of grouping is an increase in diet quality for the larvae. Increase larval activities, like ingesting humus or burrowing, increase symbiotic microorganism activity in the nearby soil. This increase in symbiotic microorganism activity creates a more nutritious diet for the larvae to consume. Two potential costs of group living in these larvae include increased risk of fungal epidemic and increased risk of predation.[16] Metarhizium fungal infection is lethal for A. dichotoma larvae, and grouping together leaves the larvae more susceptible to infection. Mogera imaizumii are able to quickly detect and consume these larvae, so living together leave them more vulnerable to being consumed.[16]

Burrowing

A. dichotoma larvae remain buried in the soil until they emerge to breed in the summer.[17] Despite this behavior, the shape of this beetle is not well suited for burrowing. The earthworm is an example of an organism that evolved specifically to move through the soil efficiently. One feature it developed is a small 2 mm transverse sectional diameter. The transverse sectional diameter of the A. dichomota last instar larva is around 20 mm.[17] Therefore, the transverse sectional area contributing to resistance as the larvae move through the soil is about one hundred times greater than that of the earthworm. To make up for this inefficiency, A. dichotoma developed a rotational burrowing technique. These larvae possess a C-shaped body, so they burrow into the ground by rotating and using their tails to kick soil upward. Kicking the soil up helps the larvae sink into the ground by fluidizing the soil, reducing resistance, and allowing them to burrow more effectively.[17]

Genetics

The horn found in male A. dichotoma is a well-studied example of an exaggerated trait that evolved through intrasexual selection.[4] This horn is a sexually dimorphic trait, which means there must be a sex-determination gene involved in its development and evolution.[18] The doublesex/Mab-3 related (DMRT) transcription factor family is a family of genes that are heavily involved in sexually dimorphic traits. These genes are evolutionarily conserved across many taxa, including worms, mammals, and beetles.[18] The specific variant of doublesex implicated in the Japanese rhinoceros beetle is known as Td-dsx which stands for T. dichotomus dsx homologue. During development, alternative splicing of Td-dsx results in the formation of male and female isoforms of the gene. Td-dsx expression at the horn-forming area of the head increases during the prepupal stage, resulting in horn development in male A. dichotomus.[18] Expression of male and female isoforms of Td-dsx increases during this time, but only the male isoform leads to horn growth. Knockdown of the male isoform of Td-dsx has shown to result in no horn growth in male Japanese rhinoceros beetles.[18]

Mating

Male-male interactions

A. dichotomus males fighting

A. dichotoma are well known for their male aggressive behavior. Males will often use their large horns to fight other males over territory and the access to female Japanese rhinoceros beetles.[3] These beetles will often fight on the trunks of host trees to determine who will keep or gain the territory. The goal of these fights is to uproot the opposition and either throw the other male onto their back or off the tree outright.[19] This aggressive behavior has been broken down into four stages:

Alternative reproductive behaviors

Smaller A. dichotoma can make use of alternative reproductive behaviors to circumvent horn-to-horn combat. One of these alternative behaviors has been described as "sneak-like behavior"[3] of which there are three variations. The first sneak-like behavior occurs when a male approaches another male from behind while the latter male has already assumed a mounted position on a female. The former beetle will try to use its horn to separate the latter beetle from the female. The second situation occurs when a male approaches another male while the latter male is positioned face-to-face with a female. In this case, the former male will attempt to mount the female. The third type of sneak-like behavior occurs when two males are fighting over a female and a third male attempts to mount the female.[3]

Female-female interactions

Female A. dichotoma do not possess nearly as long horns as their male counterparts, but they still possess a noticeable horn, nonetheless.[3] Although females of this species do not participate in the same shoving and throwing behaviors that the males do, they still exhibit some intrasexual aggressive behaviors.[21] Female A. dichotoma have been observed using their smaller cephalic horns to head-butt other females in the area. They do so to fight over territory and access to food. Larger females have an advantage when it comes to this fighting behavior.[21] Similarly to male A. dichotoma, smaller females developed a sneaky, non-confrontational strategy to gain access to resources and reproduce. Once defeated, smaller females will mount a larger female. The mounted female and mounting female rarely fight, and the mounting female will be able to access resources, including food and males.[21]

Physiology

Male Horn

Male and female side by side comparison

Body size and horn length are both important factors in determining the winner when male A. dichotoma fight. The male horn size is the most important factor used to predict the winner of these fights, but a larger horn is not always best.[22] A male A. dichotoma with a large body benefits from a larger horn so it can fight other males for access to females. The main reproductive strategy of these larger beetles is combat. The same is not true for smaller Japanese rhinoceros beetles which prefer less confrontational strategies like sneaking. In this case, the smaller beetle will prefer a smaller horn, so it is more mobile and better able to infiltrate the larger male’s territory while it is preoccupied. Therefore, horn length can be used as a metric for measuring the fighting ability of a male Japanese rhinoceros beetle, but it is not as useful to use as a measure of reproductive ability.[22]

Studies have shown that there is wide variation in male horn lengths, which indicates that a single horn length is not selected for over others.[22] A large horn is useful for fighting but acts as a hindrance when the beetle digs into nearby litter to hide during the day. The large horn has shown to reduce the efficiency of this digging behavior, which leaves the beetle vulnerable to predators. Larger horns also impair flight, making it more difficult for Japanese rhinoceros beetles to move closer to potential mates.[22] Other studies have shown that larger horns may be more fragile than smaller horns. Severe injuries sustained beetles with larger horns resulted in some of these beetles losing their horns, while similar injuries in beetles with smaller horns did not.[22]

Nutrition

Larval nutrition has a strong effect on overall growth in A. dichotoma.[23] Poor nutritional environments in the larval stage leads to decreased growth rate, which can prolong the larval period. A. dichotoma are univoltine and only produce one brood during the three summer months. If the larval period is extended for too long, the beetle can miss its breeding window, which would severely harm its individual fitness. Low nutrition levels in the larval stage are also correlated with decreased adult size of the eyes, wings, elytra, and wings in male and female Japanese rhinoceros beetles.[23] Interestingly, genitalia are not affected by nutrition levels. Males produced similarly sized genitalia regardless of nutrition levels in the larval stage. Mating and fertilization were similarly unaffected. Contrary to genitalia development in males, the male cephalic and thoracic horns are incredibly sensitive to larval nutrition levels. Low nutrition levels are associated with a 50% decrease in thoracic horn length and a 60% decrease in cephalic horn length.[23]

Interactions with humans

Research applications

A. dichotoma is a useful model organism for scientific research in insects. It is easy and convenient to set up a breeding system for these beetles in the laboratory. Breeding the beetles and culturing the progeny is a well-documented process. The Japanese rhinoceros beetle can also be bred using a soil-free apparatus which allows for non-invasive and uninterrupted monitoring of growth and development. These larvae are also easy to preserve because they can be kept at low temperatures to prevent pupation from occurring. This added element of control makes these beetles convenient to use for research purposes throughout the year. RNA interference protocols have also been developed for A. dichotomus, so it is easy to conduct experiments on genes of interest. This species of beetle is also very large, so large amounts of DNA and RNA can be extracted from a single beetle for use in sequencing analysis. A. dichotomus has become a particularly popular model organism because of its horn. The horn developmental pathways and mechanism have been thoroughly studied. A protein with antibacterial properties has been discovered in A. dichotomus, alongside a molecule with potential anti-prion activity. A. dichotoma has proven to be a useful model organism for research in fields including drug discovery, ethology, behavioral ecology, and evolutionary developmental biology.[4]

Use in medicine

A. dichotoma has been a popular ingredient in Chinese traditional medicine for almost 2000 years. Research has corroborated that A. dichotoma extracts have potential health benefits.[2] A study has shown that A. dichotomus larvae extract can significantly decrease the expression of genes associated with fat creation. The study implies that Japanese rhinoceros beetle larvae could function as a potential "anti-obesity… food supplement"[5] Another study discovered two proteins in A. dichotomus larva which exhibited antibacterial activity. These proteins are named A. d. coleoptericin A and B, with A. d being an abbreviation for A. dichotomus. A. d. coleoptericin A and B demonstrate significant activity against methicillin resistant Staphylococcus aureus (MRSA), a notoriously difficult strain of bacteria to treat with antibiotics.[6]

A. dichotoma larvae are known to consume rotting wood and fruits, so it is hypothesized that these larvae are capable of producing phytochemicals.[24] Phytochemicals are natural bioactive compounds that provide resistance to bacterial and viral infections. Researchers were interested in investigating the potential health benefits associated with these larvae and found that A. dichotoma extract contains moderate antioxidant properties.[24] Compounds found in the larvae extract are capable of scavenging for free oxygen radicals and prevent harmful oxidation in the body. The demand for natural substances that can reduce biological toxicity and food deterioration has risen due to synthetic alternatives causing harm to humans. The Japanese rhinoceros beetle larvae extract has potential to serve as an aforementioned natural alternative.[24]

Anti-prion activity

To this day, little is known about prion diseases. There is no cure and the mechanism by which normal proteins are converted to abnormal prion remains unknown. Substances found in the hemolymph of A. dichotoma have been shown to exhibit anti-prion activity once they are browned or heated for an extended period of time.[25] Administration of heated hemolymph has been shown to reduce abnormal prion protein levels in prion-infected cells. This compound has yet to be identified but is hypothesized to be a Maillard reaction product. Previous studies have shown that some Maillard reaction products are involved in the post-translational modification of prions. This compound in the hemolymph of A. dichotoma demonstrates strain-dependent anti-prion activity, as it only reduces prion formation in RML prion-infected cells. The discovery of anti-prion activity in A. dichotoma could serve as a branching point for future research in prion treatment.[25]

Akihabara culture

Insects are a prominent part of Japanese Akihabara culture.[26] Akihabara culture refers to Japanese comics, animation, videogames, dolls, and other related fields. The Japanese rhinoceros beetle is an iconic insect native to Japan and is found scattered throughout Akihabara culture. The beetle has been referenced in popular role-playing games like Dragon Quest, by the Square Enix Corporation. Throughout the series, three monsters were included that resemble A. dichotoma. The beetle has also been referenced in animated series as vehicles.[26] In Time Bokan, an animated children’s TV show from the late 1970s, the main characters possessed a time machine which resembled a rhinoceros beetle. This vehicle possessed the iconic cephalic horn found on male A. dichotoma and had wheels instead of legs. The beetle also appeared in Mushihimesama, a shooting-game where enemies are oversized insects. The main character befriends a large rhinoceros beetle and uses it to defeat enemy insects. Another game that featured the beetle is called Air. Air is a popular gal-gê game which features a female rhinoceros beetle.[26] Miss Misuzu Kamio, the game’s main character, befriends this beetle at the beginning of the game and brings it along with her throughout the rest of the story. A. dichotoma is an iconic insect that can be found throughout Japanese culture.[26]

References

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External links

Taxon identifiers
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