Lady of the Night may refer to:

• A woman who engages in prostitution

Plant

• Brassavola nodosa, a Mexican orchid
• Cestrum nocturnum, a West Indian plant species
• Lady of the Night cactus

Films

• Lady of the Night (1925 film), an American drama directed by Monta Bell
• Lady of the Pavements, a 1929 film released in the UK as Lady of the Night
• Lady of the Night (1986 film), an Italian film

Music

• Lady of the Night (album), an album by Donna Summer
  • "Lady of the Night" (song), the title track
• "Lady of the Night", a song by Bucks Fizz from Bucks Fizz
• "The Lady of the Night", a 1973 song by David Houston
• "Lady of the Night", a song by LGT, featured in the Hungarian film A kenguru ("The Kangaroo") (1975)

Other uses

• Lady of the Night, a title for Santa Muerte, a cult image, female deity, and folk saint in folk Catholicism and Mexican Neopaganism