Ladysmith may refer to:

• Ladysmith, KwaZulu-Natal, South Africa
• Ladysmith, British Columbia, Canada
• Ladysmith, Wisconsin, United States
• Ladysmith, New South Wales, Australia
• Ladysmith, Virginia, United States
• Ladysmith Island, Queensland, Australia, an island in Smith Islands National Park

Other

• Ladysmith (novel), a 1999 novel by Giles Foden
• Ladysmith Black Mambazo, a South African choral group
• Siege of Ladysmith (South Africa), 1900
• Smith & Wesson Ladysmith, a small handgun