The list of Boeing 727 operators lists both former and current operators of the aircraft.

Original commercial operators

List of operators who purchased or leased aircraft new:^[1]

Airline	Country	100	200	Notes
Aeroperú	Peru	8	9
Aerolíneas Argentinas	Argentina	1	19
Air Algérie	Algeria	2	12
Air America	United States	1
Air Canada	Canada		30
Air Charter International	France		11
Air France	France		29
Air Jamaica	Jamaica	1	11
Air Vietnam	South Vietnam	2
Airlift International	United States	5
Alaska Airlines	United States	32	29
Alia Royal Jordanian Airlines	Jordan		7
Alitalia	Italy		18
All Nippon Airways	Japan	13	33
Allegheny Airlines	United States	10	4	Rebranded as USAir in 1979
American Airlines	United States	59	125
American Flyers Airline	United States	3
Angola Air Charter	Angola	8
Ansett Australia	Australia	6	19	Ceased operations in 2002
Ariana Afghan Airlines	Afghanistan	9	3
Avensa	Venezuela	11	13
Avianca	Colombia	33	18
Braniff International Airways	United States	48	79	Ceased operations in 1982
British West Indian Airways	Trinidad and Tobago	4
Canadian Pacific Air Lines	Canada	4	2
China Airlines	Taiwan	4
Condor	Germany	7	8
Continental Airlines	United States	26	109
Cruzeiro do Sul	Brazil	2
Delta Air Lines	United States	8	183
Dominicana de Aviación	Dominican Republic	5	9	Ceased operations in 1999
Eastern Air Lines	United States	75	99	Launch customer of 727-100
Ethiopian Airlines	Ethiopia		4
Faucett Perú	Peru	5	3
FedEx Express	United States	83	60
Frontier Airlines	United States	7	3
Hapag-Lloyd Flug	Germany	8	3
Hughes Airwest	United States	3	11
Iberia	Spain		48
Icelandair	Iceland	5	2
Iran Air	Iran	6	3
Iraqi Airways	Iraq		12
Japan Airlines	Japan	23
Japan Domestic Airlines	Japan	2
JAT Yugoslav Airlines	Yugoslavia	2	18
Kuwait Airways	Kuwait		4
LACSA	Costa Rica	3	5
LAN-Chile	Chile	5
Libyan Arab Airlines	Libya		15
Lloyd Aéreo Boliviano	Bolivia	3	4	Ceased operations in 2010
Lufthansa	Germany	27	30
Mexicana de Aviación	Mexico	17	51
National Airlines	United States	21	27	Merged with Pan Am in 1980
Nigeria Airways	Nigeria	1	4
Northeast Airlines	United States	8	13	Merged with Delta Air Lines in 1972
Northwest Airlines	United States	36	82	Launch customer of 727-200
Olympic Airways	Greece	2	10
Ozark Air Lines	United States		2	Never used and sold to Pan Am
Pacific Air Lines	United States	6		Became Air West in 1968
Pacific Southwest Airlines	United States	16	33
Pan American World Airways	United States	46	105	Ceased operations in 1991
Republic Airlines	United States		22	Merged with Northwest Airlines in 1986
Royal Air Maroc	Morocco		9
PLUNA	Uruguay	3
Sabena	Belgium	6	1
Singapore Airlines	Singapore		6
Sterling Airways	Denmark		28
Southwest Airlines	United States		6	Leased from Braniff International and People Express Airlines
South African Airways	South Africa	9
Syrian Air	Syria	3
TAAG Angola Airlines	Angola	1
TAP Air Portugal	Portugal	9	8	Included aircraft operated by wholly owned subsidiary Air Atlantis
Transair Sweden	Sweden	4
Trans Australia Airlines	Australia	7	15
Trans Caribbean Airways	United States	3	2	Acquired by American Airlines in 1971
Trans International Airlines	United States	2
Trans World Airlines	United States	35	61
Tunisair	Tunisia	1	20
Turkish Airlines	Turkey		12
United Airlines	United States	126	104
Varig	Brazil	11
VASP	Brazil	2	13	Ceased operations in 2005
Wardair Canada	Canada	1
Western Airlines	United States		48	Merged with Delta Air Lines in 1987
World Airways	United States	12	3
Yemen Airways	Yemen	3	9

Other commercial operators

List of operators who purchased or leased aircraft second-hand:

Airline	Country	100	200	Notes
ACES Colombia	Colombia	12	7	Ceased operations in 2003
Aero Costa Rica	Costa Rica	1	3	Ceased operations in 1997
Aero República	Colombia	3
Aerocar Colombia	Colombia	1	1	Ceased operations in 1995
AeroGal	Ecuador		2
AeroLatin	Venezuela	1		Ceased operations in 2000
Aerolíneas Internacionales	Mexico	2	6	Ceased operations in 2003
Aerolíneas Latinas	Venezuela	4		Renamed to AeroLatin in 1995
Aeropostal Alas de Venezuela	Venezuela		8
Aerosucre	Colombia	4	5	2 aircraft are still in service
AeroSur	Bolivia	7	13	Ceased operations in 2012
Aerotal	Colombia	6		Ceased operations in 1983
African Express Airways	Kenya		2
Air 1	United States	9	3	Ceased operations in 1985
Air Alfa	Turkey		2
Air Atlanta	United States	5
Air Class Líneas Aéreas	Uruguay		2	2 aircraft are still in service
Air Commerce	Yugoslavia		2	Leased from JAT Yugoslav Airlines
Air Dream	Cambodia		1	Ceased operations in 2007
Air Florida	United States	2	5
Air Gemini	Angola	8	1
Air Holland	Netherlands		3
Air Horizon	Togo	1		Ceased operations in 2007
Air Libya	Libya		2
Air Macau	Macau		3
Air Malta	Malta	3	7
Air Micronesia	Guam	10	5	Renamed to Continental Micronesia in 1989
Air National	United States		1	Ceased operations in 1984
Air Nauru	Nauru	3
Air Niagara	United States	3		Ceased operations in 1984
Air Panamá Internacional	Panama	8	2	Ceased operations in 1990
Air Vanuatu	Vanuatu		1
Al Rais Cargo	United Arab Emirates		2	Ceased operations in 2008
Albatros Airlines	Turkey		1	Ceased operations in 1996
Allied Air	Nigeria		3
American Trans Air	United States	15	28
Amerijet International	United States	11	22
Angola Air Charter	Angola	10
Asian Express Airlines	Australia		2	Renamed to Tasman Cargo Airlines in 2008
Associated Aviation	Nigeria		1
Astar Air Cargo	United States	2	9	Ceased operations in 2012
ATA Brasil	Brazil		1	Ceased operations in 2006
Av Atlantic	United States	1	5	Ceased operations in 1996
Avesca Colombia	Colombia	2		Ceased operations in 1995
Aviogenex^[2]^[3]	Yugoslavia		17
Azerbaijan Airlines	Azerbaijan		4
Bahamas Express	United States	1		Rebranded as Majestic Air in 1988
Balkh Airlines	Afghanistan	1
Birgenair	Turkey		1	Leased from Yemen Airways
Boğaziçi Hava Taşımacılığı	Turkey		2	Ceased operations in 1989
Bosphorus Airways	Turkey		2	Ceased operations in 1993
Boston-Maine Airways	United States		3	Ceased operations in 2008
Braniff (1983-1990)	United States		34	Ceased operations in 1989
Braniff (1991-1992)	United States	2	11	Ceased operations in 1992
Capital Cargo International Airlines	United States		15	Merged with Air Transport International in 2013
Carnival Air Lines	United States	1	10	Merged with Pan Am (1996-1998) in 1998
Cayman Airways	Cayman Islands	2	3
Chanchangi Airlines	Nigeria		8
Challenge Air Cargo	United States	1		Leased from Air Panamá Internacional
Champion Air	United States	2	22	Ceased operations in 2008
Comair	South Africa		5	Operated for British Airways
Compagnie Africaine d'Aviation	DRC		1
Continental Micronesia	Guam	1	16
Cosmos Air Cargo	Colombia	1
Custom Air Transport	United States		8	Ceased operations in 2012
Cyprus Turkish Airlines	Northern Cyprus		4
Dan-Air	United Kingdom	19	19	Merged with British Airways in 1992
DHL Airways	United States	11	9	Rebraded as Astar Air Cargo in 2003
DHL Aero Expreso	Panama		2
DHL de Guatemala	Guatemala	1
Emery Worldwide Airlines	United States	42	12
Emirates	United Arab Emirates		3
European Air Transport	Belgium	7	10
Evergreen International Airlines	United States	35
Express One International	United States	29	53
Falcon Air Express	United States		14
First Air	Canada	5	4
Fly Linhas Aéreas	Brazil		2	Ceased operations in 2003
Frontier Horizon	United States	7
Germania	Germany	2
Gulf Air Transport	United States	9	3
HeavyLift Cargo Airlines	Australia	4	3
Hewa Bora Airways	DRC	4	6
Hinduja Cargo Services	India		4	Ceased operations in 2001
Holiday Airlines	Turkey		1	Ceased operations in 1996
IFL Group	United States	3		3 aircraft are still in service
Imperial Air Cargo	South Africa		3	Ceased operations in 2014
Indonesian Airlines	Indonesia		1	Ceased operations in 2007
Intercontinental de Aviación	Colombia	1
Iran Aseman Airlines	Iran		10	Last commercial operator
Ishtar Airlines	Iraq		1	Operated by African Express Airways
Isleña Colombia	Colombia	1	3	Ceased operations in 1994
İstanbul Airlines	Turkey		11	Ceased operations in 2000
Itapemirim Transportes Aéreos	Brazil	4	2	Ceased operations in 2000
Jatayu Airlines	Indonesia		8
JD Valenciana de Aviación	Venezuela		2	Ceased operations in 1994
Jetair	Germany	2		Ceased operations in 1985
K-Mile Air	Thailand		3
Kabo Air	Nigeria	7	8
Kalitta Charters	United States	4	14	6 aircraft are still in service
Kam Air	Afghanistan	1	5
Key Airlines	United States	18	4	Ceased operations in 1993
KF Cargo	Canada	14	29
Kiwi International Air Lines	United States		22	Ceased operations in 1999
Korean Air	South Korea	5	12
Ladeco	Chile	6	20	Merged with LAN-Chile in 1998
LASER Airlines	Venezuela		5
Líneas Aéreas Suramericanas	Colombia	3	5	2 aircraft are still in service
Majestic Air	United States	1		Renamed to Carnival Air Lines in 1989
Mali Air Transport	Mali		1	Operated by the Government of Mali
Mandala Airlines	Indonesia		2
McClain Airlines	United States	4		Ceased operations in 1987
Megantara Air	Indonesia		1	Ceased operations in 2009
Merpati Nusantara Airlines	Indonesia		2
MGM Grand Air	United States	3		Rebranded as Champion Air in 2008
Nationwide Airlines	South Africa		3	Ceased operations in 2008
Nesu Air	Turkey		1	Leased from Kuwait Airways
Noble Air	Turkey		5
Northeastern International Airways	United States		5
Okada Air	Nigeria		6	Ceased operations in 1997
Oman Air	Oman	1	1
Pacific East Asia Cargo Airlines	Philippines	1	1	Ceased operations in 2010
Pacific Interstate Airlines	United States	1		Renamed to Pacific Inter Air in 1985
Pacific Inter Air	United States	1		Renamed to Bahamas Express in 1987
Pacific Western Airlines	Canada		2
Palestinian Airlines	Palestine		1
Pan American Airways (1998–2004)	United States		7	Ceased operations in 2004
Panagra Airways	United States		7	Ceased operations in 1999
Panavia	Panama	1	1	Ceased operations in 2006
Payam Air	Iran		2
People Express Airlines	United States		46	Merged with Continental Airlines in 1987
Philippine Airlines	Philippines	2	2
Phoenix Airways	South Africa	5	1	Ceased operations in 1995
Piedmont Airlines	United States	8	34	Merged with USAir in 1989
Planet Airways	United States	1	6
Pride Air	United States	3	6	Ceased operations in 1985
Private Jet Expeditions	United States	1	3
Polynesian Airlines	Samoa		1	Leased from Ansett Australia
Premier Airlines	Australia	1	1	Renamed to Asian Express Airlines in 1996
Qatar Airways	Qatar		6
Quebecair	Canada	1
Raya Airways	Malaysia		3
Reeve Aleutian Airways	United States	2
Regent Air	United States	3
Rio Linhas Aéreas	Brazil		5	Ceased operations in 2017
Royal Brunei Airlines	Brunei		1
Royal Khmer Airlines	Cambodia		1	Ceased operations in 2007
Royal Nepal Airlines	Nepal	5
SAETA	Ecuador	2	3	Ceased operations in 2000
SAM Colombia	Colombia	14	6
SAN Ecuador	Ecuador	2	1	Ceased operations in 1999
Santa Bárbara Airlines	Venezuela		2
Scibe Airlift	Republic of the Congo	3		Ceased operations in 1998
Servivensa	Venezuela	3	1	Ceased operations in 2003
Sideral Linhas Aéreas	Brazil		2	Leased from Rio Linhas Aéreas
SKA Air and Logistics Uganda	Uganda		1	1 aircraft is still in service
Skybus Airlines (1985)	United States	3	4
SNAS Aviation	Saudi Arabia		5	Ceased operations in 2014
Sol Air	Honduras		2	Leased from Falcon Air Express
SonAir	Angola	1
Sultan Air	Turkey		2	Ceased operations in 1993
Sun Country Airlines	United States		42
TAESA Lineas Aéreas	Mexico	14	2	Ceased operations in 2000
TAF Linhas Aéreas	Brazil		5	Ceased operations in 2009
Talia Airways	Turkey		2	Ceased operations in 1988
TAME	Ecuador	3	6
Tasman Cargo Airlines	Australia		1
Tayfunair	Turkey		1
Teebah Airlines	Jordan		1	Operated by Iraqi Airways
Trans Air Congo	Republic of the Congo	3	1
TransAer International Airlines	Ireland		1
Transafrik International	Angola	14		3 aircraft are still in service
Transbrasil	Brazil	22
Transmile Air Services	Malaysia		17	Rebranded as Raya Airways in 2014
Tri-MG Intra Asia Airlines	Indonesia	1	1
Trump Shuttle	United States	8	17	Acquired by US Airways Shuttle in 1992
Top Air	Turkey		4
Toros Air	Turkey	1	1	Ceased operations in 1989
Total Linhas Aéreas	Brazil		6	5 aircraft are still in service
TUR European Airways	Turkey	4	4	Ceased operations in 1994
UltrAir	United States		8	Ceased operations in 1994
UPS Airlines	United States	67	8
US Airways	United States		7
US Airways Shuttle	United States		12
USAir	United States	12	64	Renamed to US Airways in 1995
Varig Logística	Brazil	2	4	Ceased operations in 2012
Viasa	Venezuela		7	Ceased operations in 1997
Vietnam Airlines	Vietnam		1
Vensecar Internacional	Venezuela	3	3
Western Pacific Airlines	United States		2	Leased from Express One International
Wien Air Alaska	United States	6	1
Wimbi Dira Airways	DRC		1
Zuliana de Aviación	Venezuela		2

Government, military and other operators

As well as commercial operators the 727 has been used by military, government and private operators. The United States military used the 727 as a military transport, designated as the C-22.