NCAA
Division I
Appalachian State
Campbell
Charlotte
Davidson
Duke
East Carolina
Elon
Gardner-Webb
High Point
NC State
North Carolina
North Carolina A&T
NC Central
Queens
UNC Asheville
UNC Greensboro
UNC Wilmington
Wake Forest
Western Carolina
Full NCAA Division I member colleges in North Carolina.
– FBS Football, 
– FCS Football, 
– Non-football
– FBS Football, 
– FCS Football, 
– Non-football
- ^ a b c Campbell, Elon, and North Carolina A&T's football teams all compete in CAA Football, the technically separate football league operated by the all-sports CAA.
- ^ Davidson's football team competes in the Pioneer Football League.
- ^ As of the 2023–24 academic year, Queens is in the second year of a four-year transition to Division I and will become an active Division I member in 2026–27.
Division II
Barton
Belmont Abbey
Catawba
Chowan
Elizabeth City State
Fayetteville State
Johnson C. Smith
Lees–McRae
Lenoir–Rhyne
Livingstone
Mars Hill
Mount Olive
St. Augustine's
Shaw
UNC Pembroke
Wingate
Winston-Salem State
Full NCAA Division II member colleges in North Carolina.
Football, – Non-football
Football, – Non-football- ^ Barton's football team competes in the South Atlantic Conference. In 2025, it will move to Conference Carolinas.
- ^ Chowan's football team competes in the Gulf South Conference. In 2025, it will move to Conference Carolinas.
- ^ a b Shaw's soccer teams compete as independents.
- ^ UNC Pembroke's football team competes in the Mountain East Conference. In 2025, it will move to Conference Carolinas.
Division III
- ^ As of the 2023–24 academic year, Warren Wilson is in the third year of a three-year transition to Division III and will become an active Division III member in 2024–25.
