This is a list of museums, galleries and interpretive centers in the Louisville metropolitan area.

Art

• 21c Museum Hotel
• Carnegie Center for Art & History (New Albany, Indiana)
• KMAC Museum
• Speed Art Museum

Regional history

• Falls of the Ohio State Park interpretive center, a museum covering the natural history related to findings in the nearby exposed Devonian fossil beds as well as the human history of the Louisville area
• The Filson Historical Society, features a museum and extensive historical collections, currently undergoing major expansion
• Frazier History Museum
• Historic Locust Grove Visitors Center, which includes a museum
• Howard Steamboat Museum (Jeffersonville, Indiana)
• Kentucky Derby Museum
• Kentucky Railway Museum (New Haven)
• Louisville Slugger Museum & Factory
• My Old Kentucky Home State Park (Bardstown)
• Portland Museum
• Riverside, The Farnsley-Moremen Landing Visitors Center, which includes a museum
• Thomas Edison House
• Whitney Young Birthplace and Museum

Bourbon

• Evan Williams Bourbon Experience,^[1] located on Louisville's Whiskey Row, featuring bourbon history and tastings, and interprets Louisville's wharf history in the 1790s
• Heaven Hill Distilleries Bourbon Heritage Center^[2] (Bardstown)
• Jim Beam American Stillhouse^[3] (Clermont)
• Oscar Getz Museum of Whiskey History (Bardstown)
• Stitzel-Weller Distillery^[4] (Shively)

Cities

• Bardstown Historical Museum (Bardstown)
• Corydon Capitol State Historic Site (Corydon, Indiana)
• Historic Middletown Museum^[5]
• Jeffersontown Historical Museum^[6] (Jeffersontown)

Counties

• The Bullitt County History Museum^[7] (Shepherdsville)
• Clark County Museum^[8] (Jeffersonville, Indiana)
• Henry County Historical Society^[9] (New Castle)
• Oldham County History Center^[10] (La Grange)

More regional historical collections can be found at the Louisville Free Public Library and the University of Louisville.

U.S. and world history

• Museum of the American Printing House for the Blind
• Civil War Museum (Bardstown), including the Civil War Museum of the Western Theater, Pioneer Village, Women's Civil War Museum, War Memorial of Mid America and the Wildlife Museum
• John Hay Center
• Louisville Slugger Museum & Factory, showcases the history of the Louisville Slugger and baseball in general
• National Society of the Sons of the American Revolution, features a historical museum and a genealogical collection
• General George Patton Museum of Leadership (Fort Knox)

Other subjects

• Kentucky Science Center, hands-on science museum featuring a four-story digital theater
• Louisville WaterWorks Museum, located at the Louisville Water Tower
• Muhammad Ali Center
• Schimpff's Candy Museum^[11] (Jeffersonville)
• Thomas Merton Center