This is a list of sites and peoples visited by the Hernando de Soto Expedition in the years 1539–1543. In May 1539, de Soto left Havana, Cuba, with nine ships, over 620 men and 220 surviving horses and landed at Charlotte Harbor, Florida. This began his three-year odyssey through the Southeastern North American continent, from which de Soto and a large portion of his men would not return.
They met many varied Native American groups, most of them bands and chiefdoms related to the widespread Mississippian culture. Only a few of these ancestral cultures survived into the seventeenth century, or their descendants combined as historic tribes known to later Europeans. Others have been recorded only in the written historical accounts of de Soto's expedition.