Lois Wilson may refer to:

• Lois W. also known as Lois Wilson (née Burnham) (1891–1988), American co-founder of Al-Anon and wife of Alcoholics Anonymous founder Bill W.
• Lois Wilson (actress) (1894–1988), actress in silent films
• Lois Miriam Wilson (born 1927), first female Moderator of the United Church of Canada, 1980–1982
• Lois Wilson (Doctors), a fictional character from the British soap opera Doctors
• Lois Wilson Langhorst (née Lois Wilson; 1914–1989), American modernist architect

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