Matlacha Pass National Wildlife Refuge
IUCN category V (protected landscape/seascape)
Map showing the location of Matlacha Pass National Wildlife Refuge
LocationLee County, Florida, United States
Nearest cityFort Myers, Florida
Coordinates26°33′33″N 82°04′17″W / 26.55917°N 82.07139°W / 26.55917; -82.07139Coordinates: 26°33′33″N 82°04′17″W / 26.55917°N 82.07139°W / 26.55917; -82.07139
Area538 acres (2.2 km2)
EstablishedSeptember 26, 1908
Governing bodyU.S. Fish and Wildlife Service
WebsiteMatlacha Pass National Wildlife Refuge

The Matlacha Pass National Wildlife Refuge is part of the United States National Wildlife Refuge System, located within the Matlacha Pass estuary, approximately 8 miles northwest of Cape Coral, Florida. The 538 acre (2.2 km2) refuge was established on September 26, 1908. It is administered as part of the J. N. "Ding" Darling National Wildlife Refuge Complex.


Trees that can be seen here include red mangrove (Rhizophora mangle), black mangrove (Avicennia germinans), white mangrove (Laguncularia racemosa), buttonwood (Conocarpus erectus) and cabbage palm (Sabal palmetto). Also found in the area are tropical species such as seagrape (Coccoloba uvifera), Florida strangler fig (Ficus aurea) and gumbo limbo (Bursera simaruba).


Some of the threatened or endangered animal species which make the refuge home are the West Indian manatee, crocodile, eastern indigo snake, wood stork and bald eagle.