Mayrhofer is a German surname meaning "from the region of Mayrhof" in Austria. Notable people with the surname include:

• Carl Mayrhofer (1837–82), Austrian obstetrician
• Johann Mayrhofer (1787–1836), Austrian poet and librettist known for his friendship with Franz Schubert
• Manfred Mayrhofer (1926–2011), Austrian linguist who specialized in Indo-Iranian languages
• Wolfgang Mayrhofer (born 1958), Austrian sailor who competed in the 1980 Olympic Olympics, academic in the field of management and organisational behaviour

Other uses

• 1690 Mayrhofer, an asteroid named after Austrian amateur astronomer Karl Mayrhofer (1903–82)