NUP214
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2OIT, 3FHC, 3FMO, 3FMP, 5DIS
Identifiers
Aliases	NUP214, CAIN, CAN, D9S46E, N214, p250, nucleoporin 214kDa, nucleoporin 214, IIAE9
External IDs	OMIM: 114350 MGI: 1095411 HomoloGene: 38008 GeneCards: NUP214
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q34.13 Start 131,125,573 bp[1] End 131,234,663 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2 B|2 21.97 cM Start 31,864,448 bp[2] End 31,943,987 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte blood bone marrow cells sural nerve spleen ganglionic eminence periodontal fiber sperm right uterine tube skin of abdomen Top expressed in secondary oocyte morula blastocyst endocardial cushion spermatid yolk sac lip neural tube spermatocyte thymus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nuclear export signal receptor activity protein binding nuclear localization sequence binding structural constituent of nuclear pore Cellular component cytosol intracellular membrane-bounded organelle focal adhesion nucleoplasm nucleus cytoplasmic side of nuclear pore nuclear pore nuclear basket nuclear pore central transport channel nuclear pore nuclear envelope host cell Biological process mRNA transport regulation of mRNA stability regulation of cell cycle protein export from nucleus protein transport viral process protein import into nucleus RNA export from nucleus mRNA export from nucleus regulation of nucleocytoplasmic transport mitotic cell cycle regulation of glycolytic process tRNA export from nucleus protein sumoylation viral transcription regulation of gene silencing by miRNA intracellular transport of virus regulation of cellular response to heat Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8021 227720 Ensembl ENSG00000126883 ENSMUSG00000001855 UniProt P35658 Q80U93 RefSeq (mRNA) NM_005085 NM_001318324 NM_001318325 NM_172268 RefSeq (protein) NP_001305253 NP_001305254 NP_005076 NP_758472 NP_001395026 Location (UCSC) Chr 9: 131.13 – 131.23 Mb Chr 2: 31.86 – 31.94 Mb PubMed search [3] [4]
Wikidata
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Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene.^[5][6][7]

Function

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome.^[7]

Structure

The structure of the N-terminal domain of Nup214 reveals a sevenbladed beta-propeller fold followed by a 30-residue C-terminal extended peptide segment (CTE). The CTE folds back onto the beta propeller and binds to its bottom face.^[8] The structure of the Nup214 NTD bound to the helicase Ddx19 in its ADP-bound state reveals the molecular basis for the interaction between the two proteins. A conserved residue of Ddx19 is shown to be crucial for complex formation in vitro and in vivo. Strikingly, the interaction surfaces exhibit strongly opposing surface potentials, with the helicase surface being positively and the Nup214 surface being negatively charged. Ddx19 is shown to bind RNA only in its ATP-bound state, and the binding of RNA and the Nup214 NTD is mutually exclusive.^[9]

Interactions

NUP214 has been shown to interact with:

• DDX19,^[9]
• CRM1
• NXF1,^[10][11] and
• NXF2,^[10] and
• ZFP36.^[12]