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((Short description|Medium for growing microorganisms))
((more cn|date=August 2022))

[[File:K pneumoniae M morganii providencia styphimuriuma.JPG|thumb|[[Streaking (microbiology)|Streak plate]]s of several bacterial species on nutrient agar plates]]

'''Nutrient agar''' is a general-purpose solid [[Growth medium|medium]] supporting growth of a wide range of non-[[Fastidious organism|fastidious]] organisms. It typically contains ([[Mass concentration (chemistry)|mass/volume]]):<ref>((cite book |title=Standard Methods for the Examination of Water and Sewage |last=American Public Health Association, American Chemical Society, Association of Official Agricultural Chemists|year=1920 |publisher=American public health association |pages=[https://archive.org/details/standardmethods01usgoog/page/n103 95] |url=https://archive.org/details/standardmethods01usgoog ))</ref>
* 0.5% [[peptone]] - this provides organic nitrogen
* 0.3% [[beef extract]]/[[yeast extract]] - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts
* 1.5% [[agar]] - this gives the mixture solidity 
* 0.5% [[Sodium Chloride|sodium chloride]] - this gives the mixture proportions similar to those found in the [[cytoplasm]] of most organisms
* [[distilled water]] - water serves as a transport medium for the agar's various substances
* pH adjusted to neutral (6.8) at ((convert|25|C|F)).

Nutrient broth has the same composition, but lacks agar.<ref>((Cite encyclopedia|title=Nutrient broth - Bugwoodwiki|url=https://wiki.bugwood.org/Nutrient_broth|access-date=2022-08-13|website=wiki.bugwood.org))</ref><ref>((Cite book|last1=Fahy|first1=P. C.|url=https://books.google.com/books?id=FxtcPwAACAAJ|title=Plant Bacterial Diseases: A Diagnostic Guide|last2=Persley|first2=G. J.|date=1983|oclc=10249741|publisher=Academic Press|isbn=9781224766033))</ref>

These ingredients are combined and boiled for approximately one minute to ensure they are mixed and    then sterilized by autoclaving, typically at ((convert|121|C|F)) for 15 minutes. Then they are cooled to around ((convert|50|C|F)) and poured into Petri dishes which are covered immediately.  Once the dishes hold solidified agar, they are stored upside down and are often refrigerated until used.  [[Inoculation]] takes place on warm dishes rather than cool ones: if refrigerated for storage, the dishes must be rewarmed to room temperature prior to inoculation.((cn|date=August 2022))

==See also==
*[[Plate count agar]]
*[[Bacteria]]
*[[Bacterial growth]]

==References==
((Reflist))
==Further reading==
*Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic Press, London.
*MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins, Baltimore.
*Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C.
*American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.

((Growth media))

[[Category:Microbiological media]]


((Microbiology-stub))

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