PRPF4B

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
4IAN, 4IFC, 4IIR, 4IJP

Identifiers

Aliases

PRPF4B, PR4H, PRP4, PRP4H, PRP4K, dJ1013A10.1, pre-mRNA processing factor 4B

External IDs

OMIM: 602338 MGI: 109584 HomoloGene: 134085 GeneCards: PRPF4B

Gene location (Human)

Chr.	Chromosome 6 (human)^[1]

Band	6p25.2	Start	4,021,267 bp^[1]
Band	6p25.2	End	4,064,983 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 13 (mouse)^[2]

Band	13\|13 A3.3	Start	34,875,302 bp^[2]
Band	13\|13 A3.3	End	34,906,064 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

endothelial cell

Achilles tendon

right uterine tube

gastric mucosa

lactiferous duct

endometrium

lymph node

tibial nerve

rectum

body of pancreas

Top expressed in

fossa

condyle

Paneth cell

cerebellar vermis

substantia nigra

otic placode

saccule

medullary collecting duct

ciliary body

dermis

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	kinase activity transferase activity protein kinase activity protein serine/threonine kinase activity nucleotide binding protein binding ATP binding cyclin-dependent protein serine/threonine kinase activity RNA binding
Cellular component	catalytic step 2 spliceosome spliceosomal complex nucleus chromosome nuclear speck
Biological process	mRNA splicing, via spliceosome protein phosphorylation mRNA processing RNA splicing phosphorylation
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


8899


19134

Ensembl


ENSG00000112739


ENSMUSG00000021413

UniProt


Q13523


Q61136

RefSeq (mRNA)


NM_003913 NM_176800


NM_013830

RefSeq (protein)


NP_003904


NP_038858

Location (UCSC)

Chr 6: 4.02 – 4.06 Mb

Chr 13: 34.88 – 34.91 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Serine/threonine-protein kinase PRP4 homolog is an enzyme that in humans is encoded by the PRPF4B gene.^[5]^[6]^[7]

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).^[7]

Interactions

PRPF4B has been shown to interact with Pinin.^[8]

References

Schultz SJ, Nigg EA (1994). "Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans". Cell Growth Differ. 4 (10): 821–30. PMID 8274451.
Gross T, Lützelberger M, Weigmann H, et al. (1997). "Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue". Nucleic Acids Res. 25 (5): 1028–35. doi:10.1093/nar/25.5.1028. PMC 146536. PMID 9102632.
Huang Y, Deng T, Winston BW (2000). "Characterization of hPRP4 kinase activation: potential role in signaling". Biochem. Biophys. Res. Commun. 271 (2): 456–63. doi:10.1006/bbrc.2000.2651. PMID 10799319.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
Dellaire G, Makarov EM, Cowger JJ, et al. (2002). "Mammalian PRP4 Kinase Copurifies and Interacts with Components of Both the U5 snRNP and the N-CoR Deacetylase Complexes". Mol. Cell. Biol. 22 (14): 5141–56. doi:10.1128/MCB.22.14.5141-5156.2002. PMC 139773. PMID 12077342.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Bennett EM, Lever AM, Allen JF (2004). "Human Immunodeficiency Virus Type 2 Gag Interacts Specifically with PRP4, a Serine-Threonine Kinase, and Inhibits Phosphorylation of Splicing Factor SF2". J. Virol. 78 (20): 11303–12. doi:10.1128/JVI.78.20.11303-11312.2004. PMC 521795. PMID 15452250.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
Zhang Y, Wolf-Yadlin A, Ross PL, et al. (2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules". Mol. Cell. Proteomics. 4 (9): 1240–50. doi:10.1074/mcp.M500089-MCP200. PMID 15951569.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.
Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
Huang B, Ahn YT, McPherson L, et al. (2007). "Interaction of PRP4 with Krüppel-Like Factor 13 Regulates CCL5 Transcription". J. Immunol. 178 (11): 7081–7. doi:10.4049/jimmunol.178.11.7081. PMC 2674583. PMID 17513757.

Post-transcriptional modification

Nuclear

Precursor mRNA
5′ cap formation
Polyadenylation
- CPSF
- CstF
- PAP
- PAB2
- CFI
- CFII
Poly(A)-binding protein
RNA editing
Polyuridylation

RNA splicing

Intron / Exon snRNP Spliceosome minor U1 Alternative splicing
pre-mRNA factors	PLRG1 PRPF3 PRPF4 PRPF4B PRPF6 PRPF8 PRPF18 PRPF19 PRPF31 PRPF38A PRPF38B PRPF39 PRPF40A PRPF40B

Cytosolic

5′ cap methylation
mRNA decapping
- DCP1A
- DCP1B
- DCP2
- DCPS
- EDC3
- EDC4

Interactions

References

Further reading