Queenston may refer to:

Places

• Queenston, a town in Ontario, Canada
• Queenston Road or Queenston Street, part of Ontario Highway 8
• Lewiston–Queenston Bridge or Queenston Bridge
• Queenston Formation, a geological formation of Upper Ordovician age that outcrops in Ontario and New York
• Queenston Delta, a clastic wedge of sediment deposited over eastern North America during the late Ordovician period

Military

• Battle of Queenston Heights or Battle of Queenston, a battle hear the town of Queenston
• Queenston-class, a ship class in the Joint Support Ship Project
  • HMCS Queenston, a Queenston-class naval auxiliary

Animals

• Queenston Stakes, Canadian thoroughbred race