Protein-coding gene in the species Homo sapiens
E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene.[5][6][7] A knockout in a human colorectal cancer cell line, HCT116, has also been created.[8]
Function
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.[7]
Interactions
RAD18 has been shown to interact with HLTF,[9] UBE2B[5][6] and UBE2A.[5][6]