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Cryptococcus neoformans stained with light India ink

Radiotrophic fungi are fungi that can perform the hypothetical biological process called radiosynthesis, which means using ionizing radiation as an energy source to drive metabolism. It has been claimed that radiotrophic fungi have been found in extreme environments such as in the Chernobyl Nuclear Power Plant.

Most radiotrophic fungi use melanin in some capacity to survive.[1] The process of using radiation and melanin for energy has been termed radiosynthesis, and is thought to be analogous to anaerobic respiration.[2] However, it is not known if multi-step processes such as photosynthesis or chemosynthesis are used in radiosynthesis or even if radiosynthesis exists in living organisms.

Discovery

Many fungi have been isolated from the area around the destroyed Chernobyl Nuclear Power Plant, some of which have been observed directing their growth of hyphae toward radioactive graphite from the disaster, a phenomenon called “radiotropism”.[3][4] Study has ruled out the presence of carbon as the resource attracting the fungal colonies, and in fact concluded that some fungi will preferentially grow in the direction of the source of beta and gamma ionizing radiation, but were not able to identify the biological mechanism behind this effect.[4] It has also been observed that other melanin-rich fungi were discovered in the cooling water from some other working nuclear reactors. The light-absorbing compound in the fungus cell membranes had the effect of turning the water black.[5] While there are many cases of extremophiles (organisms that can live in severe conditions such as that of the radioactive power plant), a hypothetical radiotrophic fungus would grow because of the radiation, rather than in spite of it.[6]

Further research conducted at the Albert Einstein College of Medicine showed that three melanin-containing fungi—Cladosporium sphaerospermum, Wangiella dermatitidis, and Cryptococcus neoformans—increased in biomass and accumulated acetate faster in an environment in which the radiation level was 500 times higher than in the normal environment. C. sphaerospermum in particular was chosen due to this species being found in the reactor at Chernobyl. Exposure of C. neoformans cells to these radiation levels rapidly (within 20–40 minutes of exposure) altered the chemical properties of its melanin, and increased melanin-mediated rates of electron transfer (measured as reduction of ferricyanide by NADH) three- to four-fold compared with unexposed cells. However, each culture was performed with at least limited nutrients provided to each fungus. The increase in biomass and other effects could be caused either by the cells directly deriving energy from ionizing radiation, or by the radiation allowing the cells to utilize traditional nutrients either more efficiently or more rapidly.[6]

Outside of the fungal studies, similar effects on melanin electron-transport capability were observed by the authors after exposure to non-ionizing radiation. The authors did not conclude whether light or heat radiation would have a similar effect on living fungal cells.[6]

Role of melanin

Melanins are an ancient family of dark-colored, naturally occurring pigments with radiation-shielding properties. These pigments can absorb electromagnetic radiation due to their dark color and high molecular weights; this quality suggests that melanin could help protect radiotropic fungi from ionizing radiation. It has been suggested that melanin's radiation-shielding properties are due to its ability to trap free radicals formed during radiolysis of water.[7] Melanin production is also advantageous to the fungus in that it can aid survival in many extreme environments. Examples of these environments include the Chernobyl Nuclear Power Plant, the International Space Station, and the Transantarctic Mountains. Melanin may also be able to help the fungus metabolize radiation, but more evidence and research is still needed.[1]

Comparisons with non-melanized fungi

Melanization may come at some metabolic cost to the fungal cells. In the absence of radiation, some non-melanized fungi (that had been mutated in the melanin pathway) grew faster than their melanized counterparts. Limited uptake of nutrients due to the melanin molecules in the fungal cell wall or toxic intermediates formed in melanin biosynthesis have been suggested to contribute to this phenomenon.[6] It is consistent with the observation that despite being capable of producing melanin, many fungi do not synthesize melanin constitutively (i.e., all the time), but often only in response to external stimuli or at different stages of their development.[8] The exact biochemical processes in the suggested melanin-based synthesis of organic compounds or other metabolites for fungal growth, including the chemical intermediates (such as native electron donor and acceptor molecules) in the fungal cell and the location and chemical products of this process, are unknown.

Use in human spaceflight

It is hypothesized that radiotrophic fungi could potentially be used as a shield to protect against radiation,[2] specifically in affiliation to the use of astronauts in space or other atmospheres. An experiment taking place at the International Space Station in December 2018 through January 2019 was conducted in order to test whether the use of radiotrophic fungi could aid in protection against ionizing radiation in space, as part of research efforts preceding a possible trip to Mars. This experiment used the radiotrophic strain of the fungi Cladosporium sphaerospermum.[2] The growth of this fungi and its ability to deflect the effects of ionizing radiation were studied for 30 days aboard the International Space Station. This experimental trial yielded very promising results.

The amount of radiation deflected was found to have a direct correlation to the amount of fungus. There was no difference in the reduction of ionizing radiation between the experimental and control group within the first 24 hour period; however, once the radiotrophic fungi had reached an adequate maturation, and with a 180° protection radius, it was found that amounts of ionizing radiation were significantly reduced as compared to the control group. With a 1.7 mm thick shield of melanized radiotrophic Cladosporium sphaerospermum, measurements of radiation nearing the end of the experimental trial were found to be 2.42% lower, demonstrating radiation deflecting capabilities five times that of the control group. Under circumstances in which the fungi would fully encompass an entity, radiation levels would be reduced by an estimated 4.34±0.7%.[2] Estimations indicate that approximately a 21 cm thick layer could significantly deflect the annual amount of radiation received on Mars’ surface. Limitations to the use of a radiotrophic fungi based shield include increased mass on missions. However as a viable substitute to reduce overall mass on potential Mars missions, a mixture with equal mole concentration of Martian soil, melanin, and a layer of fungi roughly 9 cm thick, could be used.[2]

See also

References

  1. ^ a b Dadachova, Ekaterina; Casadevall, Arturo (December 2008). "Ionizing Radiation: how fungi cope, adapt, and exploit with the help of melanin". Current Opinion in Microbiology. 11 (6): 525–531. doi:10.1016/j.mib.2008.09.013. ISSN 1369-5274. PMC 2677413. PMID 18848901.
  2. ^ a b c d e Shunk, Graham K.; Gomez, Xavier R.; Averesch, Nils J. H. (2020-07-17). "A Self-Replicating Radiation-Shield for Human Deep-Space Exploration: Radiotrophic Fungi can Attenuate Ionizing Radiation aboard the International Space Station". bioRxiv 10.1101/2020.07.16.205534.
  3. ^ Bland, J.; Gribble, L. A.; Hamel, M. C.; Wright, J. B.; Moormann, G.; Bachand, M.; Wright, G.; Bachand, G. D. (2022). "Evaluating changes in growth and pigmentation of Cladosporium cladosporioides and Paecilomyces variotii in response to gamma and ultraviolet irradiation". Scientific Reports. 12 (1): 12142. Bibcode:2022NatSR..1212142B. doi:10.1038/s41598-022-16063-z. PMC 9287308. PMID 35840596.
  4. ^ a b Zhdanova, Nelli N.; Tugay, Tatyana; Dighton, John; Zheltonozhsky, Victor; McDermott, Patrick (September 2004). "Ionizing radiation attracts soil fungi". Mycological Research. Vol. 108, no. Pt 9. pp. 1089–1096. doi:10.1017/s0953756204000966. ISSN 0953-7562. PMID 15506020. Retrieved 2023-04-11.
  5. ^ Castelvecchi, Davide (May 26, 2007). "Dark Power: Pigment seems to put radiation to good use". Science News. Vol. 171, no. 21. p. 325. Archived from the original on 2008-04-24.
  6. ^ a b c d Dadachova E, Bryan RA, Huang X, Moadel T, Schweitzer AD, Aisen P, Nosanchuk JD, Casadevall A (2007). Rutherford J (ed.). "Ionizing radiation changes the electronic properties of melanin and enhances the growth of melanized fungi". PLOS ONE. 2 (5): e457. Bibcode:2007PLoSO...2..457D. doi:10.1371/journal.pone.0000457. PMC 1866175. PMID 17520016.
  7. ^ Gessler, N. N.; Egorova, A. S.; Belozerskaya, T. A. (2014). "Melanin pigments of fungi under extreme environmental conditions (Review)". Applied Biochemistry and Microbiology. 50 (2): 105–113. doi:10.1134/S0003683814020094. ISSN 0003-6838 – via Springer.
  8. ^ Calvo AM, Wilson RA, Bok JW, Keller NP (2002). "Relationship between secondary metabolism and fungal development". Microbiol Mol Biol Rev. 66 (3): 447–459. doi:10.1128/MMBR.66.3.447-459.2002. PMC 120793. PMID 12208999.