Río Negro (Spanish and Portuguese, 'Black River') may refer to:

Rivers

Brazil

• Rio Negro (Amazon), tributary of the Amazon River
• Rio Negro (Mato Grosso do Sul)
• Rio Negro (Paraná)
• Rio Negro (Rio de Janeiro)
• Rio Negro (Rondônia)
• Rio Negro (Tocantins)

South America

• Río Negro (Argentina), in Patagonia
• Río Negro (Los Lagos), in Southern Chile
• Río Negro (Uruguay), in Rio Grande do Sul, Brazil and Uruguay, a tributary of the Uruguay River.
• Río Negro (Chaco Province), in Argentina, tributary of the Paraná River

Central America

• Río Negro (Central America), forming part of the border between Honduras and Nicaragua
• Chixoy River, also known as the Río Negro, in Guatemala

Political divisions

South America

• Río Negro Province, Argentina
• Rio Negro, Paraná, a municipality in Brazil
• Rio Negro, Mato Grosso do Sul, a municipality in Brazil
• Río Negro, Chile, a city and commune in Osorno Province
• Río Negro, Palena, a town in Palena Province, Chile
• Rionegro, a city and municipality in Colombia
• Rionegro, Santander, a town and municipality in Colombia
• Rionegro Province, Colombia
• Río Negro Department, Uruguay
• Río Negro Municipality, Venezuela

Other continents

• Río Negro, Guatemala, a city

Other

• Río Negro (newspaper), published in Argentina
• Atlético Rio Negro Clube, a Brazilian football team from Manaus, Amazonas state
• Rionegro (TransMilenio) a TransMilenio transportation system station in Bogotá, Colombia
• Río Negro Massacre, Guatemala
• Rio Negro tuco-tuco, a species of rodent
• Río Negro National Park in Paraguay
• Rio Negro Brush-tailed Rat, a species of rodent
• Palácio Rio Negro, one of the official residences of the President of Brazil
• Río Negro (film), a 1976 Cuban film
• Río Negro Airport in Chile