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Pitchers
- 68 Matthew Bollenbacher
- 60 Josue Carmona
- 48 Leodarlyn Colon
- 43 Marcos Fortunato
- 15 Miguel Gomez
- 41 Cristian Jimenez
- 73 Andy Luis
- 76 Adrian Ogando
- 77 Camilo Sanchez
- 50 Travis Sykora
- 81 Jose Ulloa
- 74 Genderson Zapata
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Catchers
- 66 Jeisel Acosta
- 32 Edward De La Cruz
- 25 Ivan Murzi
- 9 Ryan Snell
Infielders
- 5 Everett Cooper III
- 13 Winder Diaz
- 7 Juan Garcia
- 18 Angel Geraldo
- 2 Blake Klassen
- 8 Misael Mojica
- 4 Nathaniel Ochoa Leyva
- 17 Zion Pettigrew
Outfielders
- 19 Tyler Baca
- 6 Jhoan Contreras
- 30 Brenner Cox
- 26 Daniel Marte
- 33 Nick Peoples
- 23 Brandon Pimentel
- 1 Enmanuel Ramirez
- 31 Eliesel Santana
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Manager
Coaches
60-day injured list
- 79 Jose Colmenares
- 37 Edward Urena
 7-day injured list
* On Washington Nationals 40-man roster
~ Development list
# Rehab assignment
∞ Reserve list
‡ Restricted list
§ Suspended list
† Temporarily inactive list
Roster updated October 7, 2023
Transactions
→ More rosters: MiLB • Florida Complex League
→ Washington Nationals minor league players
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