Tree of Knowledge may refer to:

Religion and mythology

• Tree of the knowledge of good and evil, a tree in the Garden of Eden, mentioned in the Book of Genesis
• Yggdrasil, the Norse world tree, from which Odin hung upside down in exchange for knowledge

Publications

• The Tree of Knowledge (novel), a 1911 novel by Pío Baroja
• Drvo znanja, a Croatian magazine
• Tree of Knowledge, a 1970s publication by Marshall Cavendish
• The Tree of Knowledge: The Biological Roots of Human Understanding, a 1987 book by Humberto Maturana and Francisco Varela (1987)

Films

• The Tree of Knowledge (1920 film), a 1920 silent film directed by William C. deMille
• Tree of Knowledge (film) (Kundskabens træ), a 1981 acclaimed Danish coming-of-age drama directed by Nils Malmros

Places

• Tree of Knowledge (Australia), the traditional birthplace of the Australian Labor Party
• Tree of Knowledge, Camooweal, a tree associated with knowledge exchange in Camooweal, Queensland, Australia
• Kidman's Tree of Knowledge, a tree on Glengyle Station, Bedourie, Queensland, Australia associated with pastoralist Sidney Kidman

Other uses

• Tree of knowledge (philosophy), a metaphor used by Descartes
• Tree of knowledge system, a conceptual approach to the unification of psychology theories
• The Tree of Knowledge (mural), a work of public art in Salford, England, by Alan Boyson