Misc. intro stuff
First we are going to look at core promoter elements. The core promoter is a region that is upstream of the transcription start site (TSS +1), and it contains several elements that facilitate the binding of regulatory elements. These elements form the pre-initiation complex (PIC).
Core promoters are divided into two different classes: TSS is focused over a narrow region, or it is dispersed over a broad region (50-100bp). Some core promoter elements include BRE (GC rich region that is the TFIIB recognition site. CpG dinucleotide is very important here: [GC][GC][GA]CGCC, the TATA box (TATA[AT]A[AT][AG]), and the Inr. A promoter element downstream of the TSS is called a downstream core promoter element.
Despite the TATA box being the
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