Viral shedding is the expulsion and release of virus progeny following successful reproduction during a hostcell infection. Once replication has been completed and the host cell is exhausted of all resources in making viral progeny, the viruses may begin to leave the cell by several methods.[1]
The term is variously used to refer to viral particles shedding from a single cell, from one part of the body into another,[2] and from a body into the environment, where the virus may infect another.[3]
"Budding" through the cell envelope—in effect, borrowing from the cell membrane to create the virus' own viral envelope— into extracellular space is most effective for viruses that require their own envelope. These include such viruses as HIV, HSV, SARS or smallpox. When beginning the budding process, the viral nucleocapsid cooperates with a certain region of the host cell membrane. During this interaction, the glycosylated viral envelope protein inserts itself into the cell membrane. In order to successfully bud from the host cell, the nucleocapsid of the virus must form a connection with the cytoplasmic tails of envelope proteins.[4] Though budding does not immediately destroy the host cell, this process will slowly use up the cell membrane and eventually lead to the cell's demise. This is also how antiviral responses are able to detect virus-infected cells.[5] Budding has been most extensively studied for viruses of eukaryotes. However, it has been demonstrated that viruses infecting prokaryotes of the domain Archaea also employ this mechanism of virion release.[6]
Apoptosis (cell destruction)
Virus forcing cell to undergo apoptosis to infect macrophages
Animal cells are programmed to self-destruct when they are under viral attack or damaged in some other way. By forcing the cell to undergo apoptosis or cell suicide, release of progeny into the extracellular space is possible. However, apoptosis does not necessarily result in the cell simply popping open and spilling its contents into the extracellular space. Rather, apoptosis is usually controlled and results in the cell's genome being chopped up, before apoptotic bodies of dead cell material clump off the cell to be absorbed by macrophages. This is a good way for a virus to get into macrophages either to infect them or simply travel to other tissues in the body.
Although this process is primarily used by non-enveloped viruses, enveloped viruses may also use this. HIV is an example of an enveloped virus that exploits this process for the infection of macrophages.[7]
Viruses that have envelopes that come from nuclear or endosomal membranes can leave the cell via exocytosis, in which the host cell is not destroyed.[8] Viral progeny are synthesized within the cell, and the host cell's transport system is used to enclose them in vesicles; the vesicles of virus progeny are carried to the cell membrane and then released into the extracellular space. This is used primarily by non-enveloped viruses, although enveloped viruses display this too. An example is the use of recycling viral particle receptors in the enveloped varicella-zoster virus.[9]
Shedding from one part of the body to another
This section needs expansion with: cited content on viral shedding from one part of the body to another. You can help by adding to it. (October 2021)
Shedding from a body into the environment
This section needs expansion with: cited content on viral shedding from a body into the environment. You can help by adding to it. (October 2021)
This section needs expansion. You can help by adding to it. (October 2021)
A human with a viral disease can be contagious if they are shedding virus particles, even if they are unaware of doing so. Some viruses such as HSV-2 (which produces genital herpes) can cause asymptomatic shedding and therefore spread undetected from person to person, as no fever or other hints reveal the contagious nature of the host.[10]
^Hall CB, Douglas RG, Geiman JM, Meagher MP (October 1979). "Viral shedding patterns of children with influenza B infection". The Journal of Infectious Diseases. 140 (4): 610–3. doi:10.1093/infdis/140.4.610. PMID512419.
^Pornillos O, Garrus JE, Sundquist WI (December 2002). "Mechanisms of enveloped RNA virus budding". Trends in Cell Biology. 12 (12): 569–79. doi:10.1016/s0962-8924(02)02402-9. PMID12495845.
