The Zee Cine Award Best Actor in a Negative Role is chosen by a jury organized by Zee Entertainment Enterprises, and the winner is announced at the ceremony. It awards the actor or actress in the role of an antagonist.
Kajol was the first woman to win this award.
| Wins | Recipient |
|---|---|
| 2 | Ashutosh Rana |
| 2 | Bobby Deol |
The winners are listed below:-
| Year | Winner | Film |
|---|---|---|
| 1998 | Kajol | Gupt: The Hidden Truth |
| 1999 | Ashutosh Rana | Dushman |
| 2000 | Sangharsh | |
| 2001 | Sushant Singh | Jungle |
| 2002 | Aftab Shivdasani | Kasoor |
| 2003 | Ajay Devgan | Deewangee |
| 2004 | Rahul Dev | Footpath |
| 2005 | John Abraham | Dhoom |
| 2006 | Nana Patekar | Apaharan |
| 2007 | Saif Ali Khan | Omkara |
| 2008 | Arjun Rampal | Om Shanti Om |
| 2009 | Not Held | |
| 2010 | Not Held | |
| 2011 | Ronit Roy | Udaan |
| 2012 | Prakash Raj | Singham |
| 2013 | Rishi Kapoor | Agneepath |
| 2014 | Supriya Pathak | Goliyon Ki Raasleela Ram-Leela |
| 2015 | Not Held | |
| 2016 | Nawazuddin Siddiqui | Badlapur |
| 2017 | Jim Sarbh | Neerja |
| 2018 | Raj Arjun | Secret Superstar |
| 2019 | Tabu | Andhadhun |
| 2020 | Gulshan Devaiah Tiger Shroff |
Commando 3 War |
| 2021 | Not Held | |
| 2022 | Not Held | |
| 2023 | Bobby Deol | Love Hostel |
| 2024 | Bobby Deol | Animal |