Modelos hipotéticos de conformacións de VAMP2 e intervención na ensamblaxe do complexo SNARE para a liberación de neurotransmisores
A proteína de membrana asociada a vesículas 2 (ou VAMP2, do inglés Vesicle-associated membrane protein 2) é unha proteína que nos humanos está codificada polo xeneVAMP2 do cromosoma 17.[1][2]
As sinaptobrevinas/VAMPs, sintaxinas e a proteína asociada ao sinaptosoma de 25 kD SNAP25 son os principais compoñentes dun complexo proteico implicado no atraque e/ou fusión de vesículas sinápticas coa membrana presináptica. A VAMP2 é un membro da familia da proteína de membrana asociada a vesículas (VAMP)/sinaptobrevina. Crese que a VAMP2 participa na liberación de neurotransmisores nun paso entre o atraque e a fusión. Os ratos que carecen dun xene funcional sinaptobrevina2/VAMP2 non poden sobrevivir despois do nacemento, e teñen unha transmisión sináptica drasticamente reducida, arredor do 10% do control.[3] A proteína forma un complexo estable coa sintaxina, a SNAP25, e a complexina. Tamén forma un complexo diferente coa sinaptofisina.[2]
A VAMP2 é un probable xene candidato causante da miastenia infantil familiar debido ao seu mapa de localización e porque codifica unha proteína das vesículas sinápticas do tipo da que foi implicada na patoxénese de dita miastenia.
↑Archer BT, Ozçelik T, Jahn R, Francke U, Südhof TC (Oct 1990). "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2". The Journal of Biological Chemistry265 (28): 17267–73. PMID1976629.
↑Martincic I, Peralta ME, Ngsee JK (Oct 1997). "Isolation and characterization of a dual prenylated Rab and VAMP2 receptor". The Journal of Biological Chemistry272 (43): 26991–8. PMID9341137. doi:10.1074/jbc.272.43.26991.
↑Li Y, Chin LS, Weigel C, Li L (Nov 2001). "Spring, a novel RING finger protein that regulates synaptic vesicle exocytosis". The Journal of Biological Chemistry276 (44): 40824–33. PMID11524423. doi:10.1074/jbc.M106141200.
↑ 7,07,1Chen X, Tomchick DR, Kovrigin E, Araç D, Machius M, Südhof TC, Rizo J (Jan 2002). "Three-dimensional structure of the complexin/SNARE complex". Neuron33 (3): 397–409. PMID11832227. doi:10.1016/s0896-6273(02)00583-4.
↑ 8,08,1Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, Roa M (Jun 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". Journal of Immunology164 (11): 5850–7. PMID10820264. doi:10.4049/jimmunol.164.11.5850.
↑Imai A, Nashida T, Yoshie S, Shimomura H (Aug 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Archives of Oral Biology48 (8): 597–604. PMID12828989. doi:10.1016/s0003-9969(03)00116-x.
↑Kawanishi M, Tamori Y, Okazawa H, Araki S, Shinoda H, Kasuga M (Mar 2000). "Role of SNAP23 in insulin-induced translocation of GLUT4 in 3T3-L1 adipocytes. Mediation of complex formation between syntaxin4 and VAMP2". The Journal of Biological Chemistry275 (11): 8240–7. PMID10713150. doi:10.1074/jbc.275.11.8240.
↑McMahon HT, Missler M, Li C, Südhof TC (Oct 1995). "Complexins: cytosolic proteins that regulate SNAP receptor function". Cell83 (1): 111–9. PMID7553862. doi:10.1016/0092-8674(95)90239-2.
↑Pérez-Brangulí F, Muhaisen A, Blasi J (Jun 2002). "Munc 18a binding to syntaxin 1A and 1B isoforms defines its localization at the plasma membrane and blocks SNARE assembly in a three-hybrid system assay". Molecular and Cellular Neurosciences20 (2): 169–80. PMID12093152. doi:10.1006/mcne.2002.1122.
↑Margittai M, Otto H, Jahn R (Mar 1999). "A stable interaction between syntaxin 1a and synaptobrevin 2 mediated by their transmembrane domains". FEBS Letters446 (1): 40–4. PMID10100611. doi:10.1016/s0014-5793(99)00028-9.
↑Mollinedo F, Martín-Martín B, Calafat J, Nabokina SM, Lazo PA (Jan 2003). "Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils". Journal of Immunology170 (2): 1034–42. PMID12517971. doi:10.4049/jimmunol.170.2.1034.
↑Reed GL, Houng AK, Fitzgerald ML (Apr 1999). "Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion". Blood93 (8): 2617–26. PMID10194441. doi:10.1182/blood.V93.8.2617.
Brumell JH, Volchuk A, Sengelov H, Borregaard N, Cieutat AM, Bainton DF, Grinstein S, Klip A (Dec 1995). "Subcellular distribution of docking/fusion proteins in neutrophils, secretory cells with multiple exocytic compartments". Journal of Immunology155 (12): 5750–9. PMID7499863.
Chapman ER, An S, Barton N, Jahn R (Nov 1994). "SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils". The Journal of Biological Chemistry269 (44): 27427–32. PMID7961655.
Hunt JM, Bommert K, Charlton MP, Kistner A, Habermann E, Augustine GJ, Betz H (Jun 1994). "A post-docking role for synaptobrevin in synaptic vesicle fusion". Neuron12 (6): 1269–79. PMID8011337. doi:10.1016/0896-6273(94)90443-X.
Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene138 (1–2): 171–4. PMID8125298. doi:10.1016/0378-1119(94)90802-8.
Betz A, Okamoto M, Benseler F, Brose N (Jan 1997). "Direct interaction of the rat unc-13 homologue Munc13-1 with the N terminus of syntaxin". The Journal of Biological Chemistry272 (4): 2520–6. PMID8999968. doi:10.1074/jbc.272.4.2520.
Martincic I, Peralta ME, Ngsee JK (Oct 1997). "Isolation and characterization of a dual prenylated Rab and VAMP2 receptor". The Journal of Biological Chemistry272 (43): 26991–8. PMID9341137. doi:10.1074/jbc.272.43.26991.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene200 (1–2): 149–56. PMID9373149. doi:10.1016/S0378-1119(97)00411-3.
Nishimura Y, Hayashi M, Inada H, Tanaka T (Jan 1999). "Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins". Biochemical and Biophysical Research Communications254 (1): 21–6. PMID9920726. doi:10.1006/bbrc.1998.9876.
Valdez AC, Cabaniols JP, Brown MJ, Roche PA (Mar 1999). "Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network". Journal of Cell Science. 112 112 (6): 845–54. PMID10036234.
Margittai M, Otto H, Jahn R (Mar 1999). "A stable interaction between syntaxin 1a and synaptobrevin 2 mediated by their transmembrane domains". FEBS Letters446 (1): 40–4. PMID10100611. doi:10.1016/S0014-5793(99)00028-9.
Fasshauer D, Antonin W, Margittai M, Pabst S, Jahn R (May 1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". The Journal of Biological Chemistry274 (22): 15440–6. PMID10336434. doi:10.1074/jbc.274.22.15440.