Immunohistochemistry (IHC) it involves the process of selectively identifying antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.[1][2] IHC takes its name from the roots "immuno", which simply means antibodies used in the procedure, and "histo", means. Immunohistochemistry was been conceptualized by Albert Coons.[3][4]
This involve proper tissue collection, fixation and sectioning. A solution of formalin is often used to fix tissue, but other methods may also be used.