Seven teams contested in the 2010 AFC Champions League qualifying play-off. The two winners advanced to the 2010 AFC Champions League group stage. The losers entered the 2010 AFC Cup group stage.

Bracket

East Asia

	Round 1					Final
	1	SAFFC	3
	4	Sriwijaya	0
						SAFFC (p)		0 (4)
						Muangthong United		0 (3)
	3	SHB Đà Nẵng	0
	2	Muangthong United	3

West Asia

	Round 1					Final
	1	Al-Karamah	0
	4	Al-Wahda	1
						Al-Wahda		5
						Churchill Brothers		2
	3	Churchill Brothers
	2	(bye)	-

Matches

East Asia

Semi-final Round

30 January 2010

19:30 UTC+8

Singapore Armed Forces	3 – 0	Sriwijaya
Akiyoshi 6' Martinez 64' (pen.) Sahdan 89' (pen.)	Report

Jalan Besar Stadium, Singapore

Attendance: 2,057

Referee: Ben Williams (Australia)

31 January 2010

16:00 UTC+7

SHB Đà Nẵng	0 – 3	Muangthong United
	Report	Wongsa 33' Kouakou 45' Kaewprom 81'

Chi Lăng Stadium, Da Nang

Attendance: 14,000

Referee: Subkhiddin Mohd Salleh (Malaysia)

Final Round

6 February 2010

19:30 UTC+8

Singapore Armed Forces	0 – 0 (a.e.t.)	Muangthong United
	Report
Penalties
Sahdan Wilkinson Sandberg Bennett Akiyoshi	4 – 3	Tamaphan Wongsa Winothai Zaizen Sansook

Jalan Besar Stadium, Singapore

Attendance: 2,809

Referee: Kim Dong-Jin (Korea Republic)

West Asia

Semi-final Round

30 January 2010

19:00 UTC+3

Al-Karamah	0 – 1	Al-Wahda
	Report	Khamees 72'

Khaled bin Walid Stadium, Homs

Attendance: 30,000

Referee: Valentin Kovalenko (Uzbekistan)

Final Round

6 February 2010

19:00 UTC+4

Al-Wahda	5 – 2	Churchill Brothers
Baiano 45', 65' Khamees 46' Jumaa 63' Matar 85'	Report	Ito 41' Singh 77'

Al-Nahyan Stadium, Abu Dhabi

Attendance: 3,000

Referee: Mohsen Torky (Iran)