Golden spike may refer to:

Entertainment

• The Golden Spike, an album by Sky Larkin
• The Golden Spike, a novel by Hal Ellson

Science

• Global Boundary Stratotype Section and Point, a term in geology
• Golden Spike Company, a former American space transport company

Sports

• Golden Spike award, an annual athletics award in Belgium
• Golden Spikes Award, an annual amateur baseball award in the United States
• Golden Spike Ostrava, an annual athletics event held in Ostrava, Czech Republic
• The Golden Spike, a tradition for both pregame and Man of The Match for soccer team Atlanta United FC in the United States

Transportation

• Golden spike, the ceremonial final spike which joined the rails of the First Transcontinental Railroad