Structures of P1PK blood group antigens

P1PK (formerly: P) is a human blood group system (International Society of Blood Transfusion system 003) based upon the A4GALT gene on chromosome 22. The P antigen (later renamed P1) was first described by Karl Landsteiner and Philip Levine in 1927.[1] The P1PK blood group system consists of three glycosphingolipid antigens: Pk, P1 and NOR.[2][3] In addition to glycosphingolipids, terminal Galα1→4Galβ structures are present on complex-type N-glycans.[4] The GLOB antigen (formerly P) is now the member of the separate GLOB (globoside) blood group system.

P1PK antigens

The P1PK antigens are carbohydrate antigens that include Pk (Gb3), P1 and NOR1, NORint and NOR2. All are synthesized by Gb3/CD77 synthase (α1,4-galactosyltransferase, P1/Pk synthase).[5]

The presence or absence of P1 antigen depends on the A4GALT transcript level. It was found that differential binding of transcription factors early growth response 1 (EGR1) and runt-related transcription factor 1 (RUNX1) to the SNP rs5751348[9] genomic region with the different genotypes in the A4GALT gene leads to differential activation of A4GALT expression, leading to two genotypes: P1 and P2.[10][11]

P1PK phenotypes

P1PK phenotypes are defined by reactivity to antibodies to anti-P1, anti-P, anti-Pk anti-PP1Pk. and anti-NOR antibodies.

P1PK antibodies

Interpretation of antibody panel to detect patient antibodies towards the most relevant human blood group systems, including P1Pk.

Antibody detection

Anti-P1Pk antibodies are not usually detected with routine laboratory methods. It is possible to detect them using the Donath-Landsteiner test. This test is performed on 2 vials of blood at two different temperatures: 4 °C and 37 °C (body temperature). A test is interpreted as positive only after a patient's red blood cells have been incubated at both temperatures and subsequently hemolyzed.[16]

References

  1. ^ Landsteiner, Karl; Levine, Philip (1927). "Further Observations on Individual Differences of Human Blood". Experimental Biology and Medicine. 24 (9): 941–942. doi:10.3181/00379727-24-3649. S2CID 88119106.
  2. ^ a b c Kaczmarek, Radoslaw; Buczkowska, Anna; Mikolajewicz, Katarzyna; Krotkiewski, Hubert; Czerwinski, Marcin (2014). "P1PK, GLOB, and FORS blood group systems and GLOB collection: biochemical and clinical aspects. Do we understand it all yet?". Transfusion Medicine Reviews. 28 (3): 126–136. doi:10.1016/j.tmrv.2014.04.007. PMID 24895151.
  3. ^ a b Hellberg, Åsa Hellberg (2020). "P1PK: a blood group system with an identity crisis". ISBT Science Series. 15: 40–45. doi:10.1111/voxs.12505. S2CID 201971613.
  4. ^ Szymczak-Kulus, Katarzyna; Weidler, Sascha; Bereznicka, Anna; Mikolajczyk, Krzysztof; Kaczmarek, Radoslaw; Bednarz, Bartosz; Zhang, Tao; Urbaniak, Anna; Olczak, Mariusz; Park, Enoch Y.; Majorczyk, Edyta (January 2021). "Human Gb3/CD77 synthase produces P1 glycotope-capped N-glycans, which mediate Shiga toxin 1 but not Shiga toxin 2 cell entry". Journal of Biological Chemistry. 296: 100299. doi:10.1016/j.jbc.2021.100299. ISSN 0021-9258. PMC 7949097. PMID 33460651.
  5. ^ Kaczmarek, Radoslaw; Duk, Maria; Szymczak, Katarzyna; Korchagina, Elena; Tyborowska, Jolanta; Mikolajczyk, Krzysztof; Bovin, Nicolai; Szewczyk, Boguslaw; Jaskiewicz, Ewa; Czerwinski, Marcin (2016-01-29). "Human Gb3/CD77 synthase reveals specificity toward two or four different acceptors depending on amino acid at position 211, creating Pk, P1 and NOR blood group antigens". Biochemical and Biophysical Research Communications. 470 (1): 168–174. doi:10.1016/j.bbrc.2016.01.017. ISSN 0006-291X. PMID 26773500.
  6. ^ a b c d e f Roback JD et al. AABB Technical Manual, 16th Ed. Bethesda: AABB Press, 2008.
  7. ^ Cooling LW, Walker KE, Gille T, Koerner TAW. Shiga Toxin Binds Human Platelets Via Globotriaoslyceramide (Pk antigen) and a Novel Platelet Glycosphingolipid. Infect Immun 1998; 66: 4355-66.
  8. ^ Beadling W, Cooling L. Immunohematology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 25th Ed. Philadelphia: Saunders, 2007: 618-68.
  9. ^ "dbSNP: the NCBI database of genetic variation". Retrieved 2020-08-18.
  10. ^ Yeh, Chih‐Chun; Chang, Ching‐Jin; Twu, Yuh‐Ching; Hung, Shu‐Ting; Tsai, Yi‐Jui; Liao, Jia‐Ching; Huang, Ji‐Ting; Kao, Yu‐Hsin; Lin, Sheng‐Wei; Yu, Lung‐Chih (2018). "The differential expression of the blood group P1‐A4GALT and P2‐A4GALT alleles is stimulated by the transcription factor early growth response 1". Transfusion. 58 (4): 1054–1064. doi:10.1111/trf.14515. PMID 29399809. S2CID 4730839.
  11. ^ Westman, Julia S.; Stenfelt, Linn; Vidovic, Karina; Möller, Mattias; Hellberg, Åsa; Kjellström, Sven (2018). "Allele-selective RUNX1 binding regulates P1 blood group status by transcriptional control of A4GALT". Blood. 131 (14): 1811–1818. doi:10.1182/blood-2017-08-803080. PMID 29438961.
  12. ^ "rs397514502 RefSNP Report - dbSNP - NCBI". www.ncbi.nlm.nih.gov.
  13. ^ Duk, Maria; Reinhold, Bruce B; Reinhold, Vernon N; Kusnierz-Alejska, Grazyna; Lisowska, Elwira (2001). "Structure of a neutral glycosphingolipid recognized by human antibodies in polyagglutinable erythrocytes from the rare NOR phenotype". Journal of Biological Chemistry. 276 (44): 40574–40582. doi:10.1074/jbc.M102711200. PMID 11504714.
  14. ^ Suchanowska, Anna; Kaczmarek, Radoslaw; Duk, Maria; Lukasiewicz, Jolanta; Smolarek, Dorota; Majorczyk, Edyta; Jaskiewicz, Ewa; Laskowska, Anna; Wasniowska, Kazimiera; Lisowska, Elwira; Czerwinski, Marcin (2012). "A single point mutation in the gene encoding Gb3/CD77 synthase causes a rare inherited polyagglutination syndrome". Journal of Biological Chemistry. 287 (45): 38220–38230. doi:10.1074/jbc.M112.408286. PMC 3488091. PMID 22965229.
  15. ^ Duk, Maria; Kusnierz-Alejska, Grazyna; Korchagina, Elena Y; Bovin, Nicolai B; Bochenek, Stanislawa; Lisowska, Elwira (2005). "Anti-α-galactosyl antibodies recognizing epitopes terminating with α1,4-linked galactose: human natural and mouse monoclonal anti-NOR and anti-P1 antibodies". Glycobiology. 15 (2): 109–118. doi:10.1093/oxfordjournals.glycob.a034964. PMID 15342552.
  16. ^ Mais DD. ASCP Quick Compendium of Clinical Pathology, 2nd Ed. Chicago: ASCP Press, 2009.