The 1965 All-Big Ten Conference football team consists of American football players chosen by various organizations for All-Big Ten Conference teams for the 1965 Big Ten Conference football season. Players receiving All-Big Ten honors in 1965 included six players who were also recognized as consensus All-Americans: Aaron Brown, Jim Grabowski, Bob Griese, Bubba Smith, George Webster, and Bill Yearby. Eleven players from the 1965 Michigan State Spartans football team received first- or second-team All-Big Ten honors.

Offensive selections

Quarterbacks

• Steve Juday, Michigan State (AP-1, UPI-2)
• Bob Griese, Purdue (AP-2, UPI-1)

Running backs

• Jim Grabowski, Illinois (AP-1, UPI-1 [fb])
• Clinton Jones, Michigan State (AP-1, UPI-1 [hb])
• Carl Ward, Michigan (AP-1, UPI-1 [hb])
• Tom Barrington, Ohio State (AP-2, UPI-2 [hb])
• Bob McKelvey, Northwestern (AP-2)
• Bob Apisa, Michigan State (AP-2, UPI-2 [fb])
• Ron Rector, Northwestern (UPI-2 [hb])

Ends

• Gene Washington, Michigan State (AP-1, UPI-1)
• Bob Hadrick, Purdue (AP-1, UPI-2)
• Bill Malinchak, Indiana (AP-2)
• Cas Banaszek, Northwestern (AP-2)
• Jack Clancy, Michigan (UPI-2)

Tackles

• Tom Mack, Michigan (AP-1, UPI-1)
• Karl Singer, Purdue (AP-1, UPI-1)
• Gale Gillingham, Minnesota (AP-2, UPI-2)
• Jim Burns, Northwestern (AP-2)
• Jerry West, Michigan State (UPI-2)
• Charles Kines, Michigan (UPI-2)

Guards

• John Niland, Iowa (AP-1, UPI-1)
• Doug Van Horn, Ohio State (AP-1, UPI-1)
• Paul Faust, Minnesota (AP-2, UPI-2)
• John Karpinski, Michigan State (AP-2, UPI-2)

Centers

• Larry Kaminski, Purdue (AP-1, UPI-2)
• Ray Pryor, Ohio State (UPI-1)
• Boris Dmitroff, Michigan State (AP-2)

Defensive selections

Ends

• Aaron Brown, Minnesota (AP-1, UPI-1 [offense])
• Bubba Smith, Michigan State (AP-1, UPI-1)
• David Long, Iowa (UPI-1)
• Bo Batchelder, Illinois (AP-2, UPI-2)
• Jim Long, Purdue (AP-2, UPI-2)

Tackles

• Bill Yearby, Michigan (AP-1, UPI-1)
• Jerry Shay, Purdue (AP-1, UPI-1)
• Gary Eickman, Illinois (AP-2)
• Bill Briggs, Iowa (UPI-2)

Guards

• Harold Lucas, Michigan State (AP-2 [lb], UPI-1)
• William Ridder, Ohio State (AP-2, UPI-2)

Linebackers

• Ike Kelley, Ohio State (AP-1, UPI-1)
• Don Hansen, Illinois (AP-1, UPI-1)
• Ron Goovert, Michigan State (AP-1, UPI-2)
• Jack Calcaterra, Purdue (AP-2)
• Tom Cecchini, Michigan (AP-2, UPI-2)
• Dan Hilsabeck, Iowa (UPI-2)

Defensive backs

• Rich Volk, Michigan (AP-1, UPI-1 [s])
• George Webster, Michigan State (AP-1, UPI-1 [lb])
• Ron Acks, Illinois (AP-1, UPI-2 [hb])
• John Fill, Ohio State (AP-1)
• Don Japinga, Michigan State (AP-2, UPI-1 [hb])
• Charles King, Purdue (UPI-1 [hb])
• John Charles, Purdue (AP-2, UPI-2 [hb])
• Tom Sakal, Minnesota (AP-2)
• Tom Brigham, Wisconsin (AP-2)
• Mike Buckner, Northwestern (UPI-2 [s])

Key

AP = Associated Press^[1]

UPI = United Press International^[2]

Bold = First-team selection of both the AP and UPI