This is a list of female supervillains that can be found in American comic books and associated mediums. They are a counterpart to the superheroine, just as the villain is the counterpart to the hero.

Comic books

Marvel Comics

DC Comics

Other comics

• Angelus (Image Comics)
• Animora (Mystic)
• Bomb Queen (Image Comics)
• Celestine (Image Comics)
• Dragon Lady
• Evinlea (Negation)
• Fem Paragon (Femforce)
• Ingra (The First)
• Jessica Priest (Image Comics)
• Karai
• Mai Shen (Scion)
• Mothergod (Valiant Comics)
• Sailor Galaxia (Kodansha manga)
• Rad (Femforce)
• Tiffany (Image Comics)
• Valentina (from Darna)
• Zera (Image Comics)

American Animation

2D

• Wuya (Xiaolin Showdown)^{[citation needed]}
• Baby Doll (Batman: The Animated Series only)
• Baroness (G.I. Joe)^{[citation needed][citation needed]}
• Camilla (Kim Possible)
• Cobra (Big Hero 6)
• Doctor Blight (Captain Planet)
• Eris (The Grim Adventures of Billy and Mandy)^{[citation needed]}
• Frightwig, Joey/Rojo, Forever Princess Judith and Charmcaster (Ben 10)^{[citation needed]}
• Galatea (Justice League Unlimited only)
• Greta von Gruesome (Larryboy)
• High Voltage (Big Hero 6)
• Inque (Batman Beyond only)
• Jinx (DC Comics)
• Lady Kale (Princess Gwenevere and the Jewel Riders)^{[citation needed]}
• Livewire (DC Comics)
• Momakase (Big Hero 6)
• Morgana (Princess Gwenevere and the Jewel Riders)^{[citation needed]}
• Mrs. Clayface (DC Superhero Girls)
• Penelope Spectra and Ember McLain (Danny Phantom)
• Princess Morbucks (The Powerpuff Girls)
• Sedusa and Femme Fatale (The Powerpuff Girls)
• Shego (Kim Possible)^{[citation needed]}
• Eletronique (Kim Possible)
• Supersonic Sue (Big Hero 6)
• Talon (Static Shock)^{[citation needed]}
• Yzma Woman (The Emperor's New School)
• Zarana (G.I.Joe)^{[citation needed]}

3D

• The Bad Apple (VeggieTales)
• Empress of Unhappiness (Bibleman)
• Lamprey (Shadow Raiders) Hexadecimal (ReBoot)^{[citation needed]}
• Daemon (ReBoot)^{[citation needed]}
• Evelyn Deaver (The Incredibles II)
• The Grand Duchess of Greed (Bibleman)
• Scarlet Overkill (Minions)
• The Baroness (Bibleman)

Live action TV

• Astronema (Power Rangers: Lost Galaxy) was turned to good by Zordon's energy wave.
• Azkadellia (Tin Man) was turned back to good^{[citation needed]}
• Chase (Blade: The Series)
• Christine White (The 10th Kingdom)^{[citation needed]}
• Divatox (Power Rangers: Turbo)was turned human by Zordon's energy wave
• I.M. Wonderful (Bibleman)
• Irina Derevko (Alias)^{[citation needed]}
• Ice (Amazing Extraordinary Friends)
• Madam Glitz (Bibleman)
• Raina (Cleopatra 2525)^{[citation needed]}
• The Gossip Queen (Bibleman)