Polyethylene glycol (PEG; /ˌpɒliˈɛθəlˌiːnˈɡlaɪˌkɒl,-ˈɛθɪl-,-ˌkɔːl/) is a polyether compound derived from petroleum with many applications, from industrial manufacturing to medicine. PEG is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. The structure of PEG is commonly expressed as H−(O−CH2−CH2)n−OH.[3]
Pharmaceutical-grade PEG is used as an excipient in many pharmaceutical products, in oral, topical, and parenteral dosage forms.[4]
PEG is the basis of a number of laxatives (as MiraLax, RestoraLAX, etc.).[5]Whole bowel irrigation with polyethylene glycol and added electrolytes is used for bowel preparation before surgery or colonoscopy or for children with constipation.[6]Macrogol (with brand names such as Laxido, Movicol and Miralax) is the generic name for polyethylene glycol used as a laxative. The name may be followed by a number which represents the average molecular weight (e.g. macrogol 3350, macrogol 4000 or macrogol 6000).
An example of PEG hydrogels (see Biological uses section) in a therapeutic has been theorized by Ma et al. They propose using the hydrogel to address periodontitis (gum disease) by encapsulating stem cells in the gel that promote healing in the gums.[7] The gel with encapsulated stem cells was to be injected into the site of disease and crosslinked to create the microenvironment required for the stem cells to function.
A PEGylated lipid is used as an excipient in both the Moderna and Pfizer–BioNTech vaccines for SARS-CoV-2. Both RNA vaccines consist of messenger RNA, or mRNA, encased in a bubble of oily molecules called lipids. Proprietary lipid technology is used for each. In both vaccines, the bubbles are coated with a stabilizing molecule of polyethylene glycol.[medical citation needed] PEG could trigger allergic reaction,[9] and allergic reactions are the driver for both the United Kingdom and Canadian regulators to issue an advisory, noting that: two "individuals in the U.K. ... were treated and have recovered" from anaphylactic shock.[10][11] The US CDC stated that in their jurisdiction six cases of "severe allergic reaction" had been recorded from more than 250,000 vaccinations, and of those six only one person had a "history of vaccination reactions".[12]
Chemical uses
The remains of the 16th century carrackMary Rose undergoing conservation treatment with PEG in the 1980sTerra cotta warrior, showing traces of original color
Polyethylene glycol is also commonly used as a polar stationary phase for gas chromatography, as well as a heat transfer fluid in electronic testers.
PEG is frequently used to preserve waterlogged wood and other organic artifacts that have been salvaged from underwater archaeological contexts, as was the case with the warship Vasa in Stockholm,[13] and similar cases. It replaces water in wooden objects, making the wood dimensionally stable and preventing warping or shrinking of the wood when it dries.[5] In addition, PEG is used when working with green wood as a stabilizer, and to prevent shrinkage.[14]
PEG has been used to preserve the painted colors on Terracotta Warriors unearthed at a UNESCO World Heritage site in China.[15] These painted artifacts were created during the Qin Shi Huang (first emperor of China) era. Within 15 seconds of the terra-cotta pieces being unearthed during excavations, the lacquer beneath the paint begins to curl after being exposed to the dry Xi'an air. The paint would subsequently flake off in about four minutes. The German Bavarian State Conservation Office developed a PEG preservative that when immediately applied to unearthed artifacts has aided in preserving the colors painted on the pieces of clay soldiers.[16]
PEG is often used (as an internal calibration compound) in mass spectrometry experiments, with its characteristic fragmentation pattern allowing accurate and reproducible tuning.
An example study was done using PEG-diacrylate hydrogels to recreate vascular environments with the encapsulation of endothelial cells and macrophages. This model furthered vascular disease modeling and isolated macrophage phenotype's effect on blood vessels.[19]
PEG is commonly used as a crowding agent in in vitro assays to mimic highly crowded cellular conditions.[20] Although polyethylene glycol is considered biologically inert, it can form non-covalent complexes with monovalent cations such as Na+, K+, Rb+, and Cs+, affecting equilibrium constants of biochemical reactions.[21][22]
In microbiology, PEG precipitation is used to concentrate viruses. PEG is also used to induce complete fusion (mixing of both inner and outer leaflets) in liposomes reconstituted in vitro.
Gene therapy vectors (such as viruses) can be PEG-coated to shield them from inactivation by the immune system and to de-target them from organs where they may build up and have a toxic effect.[23] The size of the PEG polymer has been shown to be important, with larger polymers achieving the best immune protection.
When working with phenol in a laboratory situation, PEG 300 can be used on phenol skin burns to deactivate any residual phenol.[27]
In biophysics, polyethylene glycols are the molecules of choice for the functioning ion channels diameter studies, because in aqueous solutions they have a spherical shape and can block ion channel conductance.[28][29]
PEG is used in a number of toothpastes[5] as a dispersant. In this application, it binds water and helps keep xanthan gum uniformly distributed throughout the toothpaste.
PEG has been used as the gate insulator in an electric double-layer transistor to induce superconductivity in an insulator.[36]
PEG is used as a polymer host for solid polymer electrolytes. Although not yet in commercial production, many groups around the globe are engaged in research on solid polymer electrolytes involving PEG, with the aim of improving their properties, and in permitting their use in batteries, electro-chromic display systems, and other products in the future.
PEG is injected into industrial processes to reduce foaming in separation equipment.
PEG is the main ingredient in the paint (known as "fill") in paintballs.
Health effects
PEG is considered biologically inert and safe by the U.S. FDA.
However, a growing body of evidence shows the existence of a detectable level of anti-PEG antibodies in approximately 72% of the population, never treated with PEGylated drugs, based on plasma samples from 1990 to 1999.[38][further explanation needed] Due to its ubiquity in a multitude of products and the large percentage of the population with antibodies to PEG, hypersensitive reactions to PEG are an increasing concern.[39][40] Allergy to PEG is usually discovered after a person has been diagnosed with an allergy to an increasing number of seemingly unrelated products, including processed foods, cosmetics, drugs, and other substances that contain PEG or were manufactured with PEG.[39]
Available forms and nomenclature
PEG, PEO, and POE refer to an oligomer or polymer of ethylene oxide. The three names are chemically synonymous, but historically PEG is preferred in the biomedical field, whereas PEO is more prevalent in the field of polymer chemistry. Because different applications require different polymer chain lengths, PEG has tended to refer to oligomers and polymers with a molecular mass below 20,000g/mol, PEO to polymers with a molecular mass above 20,000g/mol, and POE to a polymer of any molecular mass.[41] PEGs are prepared by polymerization of ethylene oxide and are commercially available over a wide range of molecular weights from 300g/mol to 10,000,000g/mol.[42]
PEG and PEO are liquids or low-melting solids, depending on their molecular weights. While PEG and PEO with different molecular weights find use in different applications, and have different physical properties (e.g. viscosity) due to chain length effects, their chemical properties are nearly identical. Different forms of PEG are also available, depending on the initiator used for the polymerization process – the most common initiator is a monofunctional methyl ether PEG, or methoxypoly(ethylene glycol), abbreviated mPEG. Lower-molecular-weight PEGs are also available as purer oligomers, referred to as monodisperse, uniform, or discrete. Very high-purity PEG has recently been shown to be crystalline, allowing determination of a crystal structure by x-ray crystallography.[42] Since purification and separation of pure oligomers is difficult, the price for this type of quality is often 10–1000 fold that of polydisperse PEG.
PEGs are also available with different geometries.
Branched PEGs have three to ten PEG chains emanating from a central core group.
Star PEGs have 10 to 100 PEG chains emanating from a central core group.
Comb PEGs have multiple PEG chains normally grafted onto a polymer backbone.
The numbers that are often included in the names of PEGs indicate their average molecular weights (e.g. a PEG with n = 9 would have an average molecular weight of approximately 400 daltons, and would be labeled PEG 400). Most PEGs include molecules with a distribution of molecular weights (i.e. they are polydisperse). The size distribution can be characterized statistically by its weight average molecular weight (Mw) and its number average molecular weight (Mn), the ratio of which is called the polydispersity index (ĐM). Mw and Mn can be measured by mass spectrometry.
PEG and related polymers (PEG phospholipid constructs) are often sonicated when used in biomedical applications. However, as reported by Murali et al., PEG is very sensitive to sonolytic degradation and PEG degradation products can be toxic to mammalian cells. It is, thus, imperative to assess potential PEG degradation to ensure that the final material does not contain undocumented contaminants that can introduce artifacts into experimental results.[44]
PEGs and methoxypolyethylene glycols are manufactured by Dow Chemical under the trade name Carbowax for industrial use, and Carbowax Sentry for food and pharmaceutical use. They vary in consistency from liquid to solid, depending on the molecular weight, as indicated by a number following the name. They are used commercially in numerous applications, including foods, in cosmetics, in pharmaceutics, in biomedicine, as dispersing agents, as solvents, in ointments, in suppository bases, as tablet excipients, and as laxatives. Some specific groups are lauromacrogols, nonoxynols, octoxynols, and poloxamers.
The production of polyethylene glycol was first reported in 1859. Both A. V. Lourenço and Charles Adolphe Wurtz independently isolated products that were polyethylene glycols.[45] Polyethylene glycol is produced by the interaction of ethylene oxide with water, ethylene glycol, or ethylene glycol oligomers.[46] The reaction is catalyzed by acidic or basic catalysts. Ethylene glycol and its oligomers are preferable as a starting material instead of water, because they allow the creation of polymers with a low polydispersity (narrow molecular weight distribution). Polymer chain length depends on the ratio of reactants.
HOCH2CH2OH + n(CH2CH2O) → HO(CH2CH2O)n+1H
Depending on the catalyst type, the mechanism of polymerization can be cationic or anionic. The anionic mechanism is preferable because it allows one to obtain PEG with a low polydispersity. Polymerization of ethylene oxide is an exothermic process. Overheating or contaminating ethylene oxide with catalysts such as alkalis or metal oxides can lead to runaway polymerization, which can end in an explosion after a few hours.
Polyethylene oxide, or high-molecular-weight polyethylene glycol, is synthesized by suspension polymerization. It is necessary to hold the growing polymer chain in solution in the course of the polycondensation process. The reaction is catalyzed by magnesium-, aluminium-, or calcium-organoelement compounds. To prevent coagulation of polymer chains from solution, chelating additives such as dimethylglyoxime are used.
PEO's have "very low singledose oral toxicity", on the order of tens of grams per kg body weight (oral).[3] Because of its low toxicity, PEO is used in a variety of edible products.[48] The polymer is used as a lubricating coating for various surfaces in aqueous and non-aqueous environments.[49]
The precursor to PEGs is ethylene oxide, which is hazardous.[50] Ethylene glycol and its ethers are nephrotoxic if applied to damaged skin.[51]
^Ma Y, Ji Y, Zhong T, Wan W, Yang Q, Li A, et al. (December 2017). "Bioprinting-Based PDLSC-ECM Screening for in Vivo Repair of Alveolar Bone Defect Using Cell-Laden, Injectable and Photocrosslinkable Hydrogels". ACS Biomaterials Science & Engineering. 3 (12): 3534–3545. doi:10.1021/acsbiomaterials.7b00601. PMID33445388.
^Breton MF, Discala F, Bacri L, Foster D, Pelta J, Oukhaled A (3 July 2013). "Exploration of Neutral Versus Polyelectrolyte Behavior of Poly(ethylene glycol)s in Alkali Ion Solutions using Single-Nanopore Recording". The Journal of Physical Chemistry Letters. 4 (13): 2202–2208. doi:10.1021/jz400938q. ISSN1948-7185.
^Harmening DM (2005). Modern Blood Banking & Transfusion Practices. F. A. Davis Company. ISBN978-0-8036-1248-8.
^Monteiro-Riviere NA, Inman AO, Jackson H, Dunn B, Dimond S (May 2001). "Efficacy of topical phenol decontamination strategies on severity of acute phenol chemical burns and dermal absorption: in vitro and in vivo studies in pig skin". Toxicology and Industrial Health. 17 (4): 95–104. doi:10.1191/0748233701th095oa. PMID12479505. S2CID46229131.
^For example, in the online catalogArchived 29 December 2006 at the Wayback Machine of Scientific Polymer Products, Inc., poly(ethylene glycol) molecular weights run up to about 20,000, while those of poly(ethylene oxide) have six or seven digits.
^Andersen FA (1999). "Special Report: Reproductive and Developmental Toxicity of Ethylene Glycol and Its Ethers". International Journal of Toxicology. 18 (3): 53–67. doi:10.1177/109158189901800208. S2CID86231595.
