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The ultrastructure of a single bacterial cell (Bacillus subtilis). The scale bar is 200 nm.

Ultrastructure (or ultra-structure) is the architecture of cells and biomaterials that is visible at higher magnifications than found on a standard optical light microscope. This traditionally meant the resolution and magnification range of a conventional transmission electron microscope (TEM) when viewing biological specimens such as cells, tissue, or organs. Ultrastructure can also be viewed with scanning electron microscopy and super-resolution microscopy, although TEM is a standard histology technique for viewing ultrastructure. Such cellular structures as organelles, which allow the cell to function properly within its specified environment, can be examined at the ultrastructural level.

Ultrastructure, along with molecular phylogeny, is a reliable phylogenetic way of classifying organisms.[1] Features of ultrastructure are used industrially to control material properties and promote biocompatibility.


In 1931, German engineers Max Knoll and Ernst Ruska invented the first electron microscope.[2] With the development and invention of this microscope, the range of observable structures that were able to be explored and analyzed increased immensely, as biologists became progressively interested in the submicroscopic organization of cells. This new area of research concerned itself with substructure, also known as the ultrastructure.[3]


Many scientists use ultrastructural observations to study the following, including but not limited to:


A common ultrastructural feature found in plant cells is the formation of calcium oxalate crystals.[9] It has been theorized that these crystals function to store calcium within the cell until it is needed for growth or development.[10]

Calcium oxalate crystals can also form in animals, and kidney stones are a form of these ultrastructural features. Theoretically, nanobacteria could be used to decrease the formation of calcium oxalate kidney stones.[11]


Controlling ultrastructure has engineering uses for controlling the behavior of cells. Cells respond readily to changes in their extracellular matrix (ECM), so manufacturing materials to mimic ECM allows for increased control over the cell cycle and protein expression.[12]

Many cells, such as plants, produce calcium oxalate crystals, and these crystals are usually considered ultrastructural components of plant cells. Calcium oxalate is a material that is used to manufacture ceramic glazes [6], and it also has biomaterial properties. For culturing cells and tissue engineering, this crystal is found in fetal bovine serum, and is an important aspect of the extracellular matrix for culturing cells.[13]  

Ultrastructure is an important factor to consider when engineering dental implants. Since these devices interface directly with bone, their incorporation to surrounding tissue is necessary to optimal device function. It has been found that applying a load to a healing dental implant allows for increased osseointegration with facial bones.[14] Analyzing the ultrastructure surrounding an implant is useful in determining how biocompatible it is and how the body reacts to it. One study found implanting granules of a biomaterial derived from pig bone caused the human body to incorporate the material into its ultrastructure and form new bone.[15]

Hydroxyapatite is a biomaterial used to interface medical devices directly to bone by ultrastructure. Grafts can be created along with 𝛃-tricalcium phosphate, and it has been observed that surrounding bone tissue with incorporate the new material into its extracellular matrix.[16] Hydroxyapatite is a highly biocompatible material, and its ultrastructural features, such as crystalline orientation, can be controlled carefully to ensure optimal biocompatibility.[17] Proper crystal fiber orientation can make introduced minerals, like hydroxyapatite, more similar to the biological materials they intend to replace. Controlling ultrastructural features makes obtaining specific material properties possible.


  1. ^ Laura Wegener Parfrey; Erika Barbero; Elyse Lasser; Micah Dunthorn; Debashish Bhattacharya; David J Patterson; Laura A Katz (December 2006). "Evaluating support for the current classification of eukaryotic diversity". PLOS Genetics. 2 (12): e220. doi:10.1371/JOURNAL.PGEN.0020220. ISSN 1553-7390. PMC 1713255. PMID 17194223. Wikidata Q21090155.
  2. ^ Masters, Barry R (March 2009). "History of the Electron Microscope in Cell Biology" (PDF). Encyclopedia of Life Sciences (ELS). Chichester: John Wiley & Sons, Ltd. doi:10.1002/9780470015902.a0021539. ISBN 9780470016176.
  3. ^ Brieger, E.M. (1963). "Ultrastructure of the Cell". Structure and Ultrastructure of Microorganisms. Elsevier. p. 1–7. doi:10.1016/b978-0-12-134350-7.50005-8. ISBN 978-0-12-134350-7.
  4. ^ Eyden, B.; Banerjee, S.S.; Ru, Y.; Liberski, P. (2014). The Ultrastructure of Human Tumours: Applications in Diagnosis and Research. Springer Berlin Heidelberg. ISBN 978-3-642-39168-2.
  5. ^ Musser, Robert L.; Thomas, Shirley A.; Wise, Robert R.; Peeler, Thomas C.; Naylor, Aubrey W. (1984-04-01). "Chloroplast Ultrastructure, Chlorophyll Fluorescence, and Pigment Composition in Chilling-Stressed Soybeans". Plant Physiology. 74 (4): 749–754. doi:10.1104/pp.74.4.749. ISSN 0032-0889. PMC 1066762. PMID 16663504.
  6. ^ Moreira, Carolina A.; Dempster, David W.; Baron, Roland (2000). "Anatomy and Ultrastructure of Bone – Histogenesis, Growth and Remodeling". Endotext. South Dartmouth (MA):, Inc. PMID 25905372.
  7. ^ Cramer, Elisabeth M.; Norol, Françoise; Guichard, Josette; Breton-Gorius, Janine; Vainchenker, William; Massé, Jean-Marc; Debili, Najet (1997-04-01). "Ultrastructure of Platelet Formation by Human Megakaryocytes Cultured With the Mpl Ligand". Blood. 89 (7): 2336–2346. doi:10.1182/blood.V89.7.2336. ISSN 1528-0020. PMID 9116277. S2CID 7757033.
  8. ^ Ferreira, Adelina; Dolder, Heidi (1990-01-06). "Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere" (PDF). Biocell. 27 (3): 353–362. ISSN 0327-9545. PMID 15002752.
  9. ^ Prychid, C. J.; Jabaily, R. S.; Rudall, P. J. (2008-03-13). "Cellular Ultrastructure and Crystal Development in Amorphophallus (Araceae)". Annals of Botany. 101 (7): 983–995. doi:10.1093/aob/mcn022. ISSN 0305-7364. PMC 2710233. PMID 18285357.
  10. ^ Tilton, V. R.; Horner, H. T. (1980). "Calcium Oxalate Raphide Crystals and Crystalliferous Idioblasts in the Carpels of Ornithogalum caudatum". Annals of Botany. 46 (5): 533–539. doi:10.1093/oxfordjournals.aob.a085951. ISSN 1095-8290.
  11. ^ Goldfarb, David S. (2004-10-19). "Microorganisms and Calcium Oxalate Stone Disease". Nephron Physiology. 98 (2): 48–54. doi:10.1159/000080264. ISSN 1660-2137. PMID 15499215. S2CID 29369994.
  12. ^ Khademhosseini, Ali (2008). Micro and nanoengineering of the cell microenvironment: technologies and applications. Boston: Artech House. ISBN 978-1-59693-149-7.
  13. ^ Pedraza, Claudio E.; Chien, Yung‐Ching; McKee, Marc D. (2008). "Calcium oxalate crystals in fetal bovine serum: Implications for cell culture, phagocytosis and biomineralization studies in vitro". Journal of Cellular Biochemistry. 103 (5): 1379–1393. doi:10.1002/jcb.21515. ISSN 0730-2312. PMID 17879965. S2CID 43217705.
  14. ^ Meyer, U.; Joos, U.; Mythili, J.; Stamm, T.; Hohoff, A.; Fillies, T.; Stratmann, U.; Wiesmann, H.P. (2004). "Ultrastructural characterization of the implant/bone interface of immediately loaded dental implants". Biomaterials. 25 (10): 1959–1967. doi:10.1016/j.biomaterials.2003.08.070. PMID 14738860.
  15. ^ Orsini, Giovanna; Scarano, Antonio; Piattelli, Maurizio; Piccirilli, Marcello; Caputi, Sergio; Piattelli, Adriano (2006). "Histologic and Ultrastructural Analysis of Regenerated Bone in Maxillary Sinus Augmentation Using a Porcine Bone–Derived Biomaterial". Journal of Periodontology. 77 (12): 1984–1990. doi:10.1902/jop.2006.060181. ISSN 0022-3492. PMID 17209782.
  16. ^ Fujita, Rumi; Yokoyama, Atsuro; Nodasaka, Yoshinobu; Kohgo, Takao; Kawasaki, Takao (2003). "Ultrastructure of ceramic-bone interface using hydroxyapatite and β-tricalcium phosphate ceramics and replacement mechanism of β-tricalcium phosphate in bone". Tissue and Cell. 35 (6): 427–440. doi:10.1016/S0040-8166(03)00067-3. PMID 14580356.
  17. ^ Zhuang, Zhi; Miki, Takuya; Yumoto, Midori; Konishi, Toshiisa; Aizawa, Mamoru (2012). "Ultrastructural Observation of Hydroxyapatite Ceramics with Preferred Orientation to a-plane Using High-resolution Transmission Electron Microscopy". Procedia Engineering. 36: 121–127. doi:10.1016/j.proeng.2012.03.019.