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![]() | Text and/or other creative content from this version of Genome engineering was copied or moved into Genome editing with this edit. The former page's history now serves to provide attribution for that content in the latter page, and it must not be deleted as long as the latter page exists. |
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This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Jez.chow. Peer reviewers: Richard.arnold32.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 21:43, 17 January 2022 (UTC)
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Hatonya.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 21:43, 17 January 2022 (UTC)
This article was the subject of a Wiki Education Foundation-supported course assignment, between 27 August 2018 and 8 December 2018. Further details are available on the course page. Student editor(s): Gaellemardy.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 21:43, 17 January 2022 (UTC)
sorry, but why is the "recombinant Adeno-Associated Virus (rAAV)" approach not mentioned in the article?
according to " http://www.labnews.co.uk/features/the-importance-of-gene-editing/ " this non-pathogenic single stranded DNA-virus [...] has a unique and powerful capability to induce HR at rates of around 1,000 times greater than seen using simple double stranded DNA vectors. rAAV vectors are engineered to contain a single stranded DNA ‘replacement’ genome that is substantially homologous to the target gene of interest and can therefore act as a template for HR
The alternative template DNA could contain any of the full range of genetic alterations (small or large gene deletions, point-mutations, reversion of mutations to wild-type, translocations, amplifications and transgene insertions), and these will be incorporated into the cell’s genome with unparalleled precision. As the vectors do not contain any viral genes, no viral genes will be co-inserted. Though there is some potential for random integration, this is minimal so most mutations are generated without introducing unwanted and confounding genotypes and/or phenotypes.
keep the great work! :) — Preceding unsigned comment added by Marcos.alberto (talk • contribs) 14:26, 18 April 2013 (UTC)
I've just added these two reviews to support the lead paragraph. However, I think there's a lot more content in this article that they could be used for, so I'm leaving this note here for anyone who wants to help.
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(help)--Arc de Ciel (talk) 07:19, 20 July 2013 (UTC)
I suggest to change the term CRISPR to CRISPR/Cas in the following sentence: "There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), CRISPR, and engineered meganuclease re-engineered homing endonucleases."
Because CRISPR refers to the origin of the guiding RNA sequences (or to this type of bacterial immune system) but CRISPR is actually not the nuclease. The nuclease is called Cas.
references e.g.: "DNA targeting specificity of RNA-guided Cas9 nucleases" Nature Biotechnology 31, 827–832 (2013) http://www.nature.com/nbt/journal/v31/n9/full/nbt.2647.html
or
"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity." Science. 2012 Aug 17;337(6096):816-21 http://www.sciencemag.org/content/337/6096/816.short --N1K0W1N (talk) 19:00, 15 December 2013 (UTC)
In the first section of this article CRISPR/Cas9 is brought us as a breakthrough in genome editing. The reader should have the ability to the click on CRISPR and be brought to the wikipedia page to learn more about what it is. If there is no link than this should be its own short section or have a description of CRISPR added to the gene therapy section. Kputnam28 (talk) 05:17, 12 February 2016 (UTC)
COI concerns have been raised w.r.t. Genome editing (and other articles - see Wikipedia:Conflict_of_interest/Noticeboard#Cellectis). It would be well for a neutral and informed editor to review this edit, from a user with declared COI acting on behalf of Cellectis, a company which has an interest in Meganuclease. --Tagishsimon (talk) 17:18, 18 March 2016 (UTC)
As a separate issue the article as a whole still needs some work:
There are plenty of good academic reviews out there, so it'd be good to try to get this article up to at least B class. I'm happy to have a go at some of it but I'd gladly welcome help. T.Shafee(Evo﹠Evo)talk 12:54, 10 April 2016 (UTC)
Homology-independent targeted integration (HITI)
This article was the subject of a Wiki Education Foundation-supported course assignment, between 21 August 2023 and 15 December 2023. Further details are available on the course page. Peer reviewers: MatthieuFoucu.
— Assignment last updated by Thecanyon (talk) 05:33, 12 December 2023 (UTC)